Journal of Applied Glycoscience
Online ISSN : 1880-7291
Print ISSN : 1344-7882
ISSN-L : 1344-7882
62 巻, 4 号
選択された号の論文の6件中1~6を表示しています
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  • Kazuhiro Nara, Yoji Kato
    2015 年 62 巻 4 号 p. 149-151
    発行日: 2015年
    公開日: 2015/11/20
    [早期公開] 公開日: 2015/06/30
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    A heterogalactan fraction, accounting for approximately 0.5% of the dry weight of the fruit body, was derived from a water-extracted polysaccharide fraction from antler-shaped Ganoderma lucidum. The heterogalactan fraction was characterized by sugar composition and sugar linkage composition analyses. The results suggest that the heterogalactan fraction consists of a fucomannogalactan (a linear (1→6)-D-galactan substituted at the O-2 position with non-reducing fucosyl and mannosyl residues), or a mixture of fucogalactan (a linear (1→6)-D-galactan substituted at the O-2 position with non-reducing fucosyl residues) and mannogalactan (a linear (1→6)-D-galactan substituted at the O-2 position with non-reducing mannosyl residues). The molecular weight of the heterogalactan fraction was estimated to be 1.0 × 104 by gel filtration on Sepharose CL-6B.
  • Takako Hirano, Rie Uehara, Haruka Shiraishi, Wataru Hakamata, Toshiyuk ...
    2015 年 62 巻 4 号 p. 153-157
    発行日: 2015年
    公開日: 2015/11/20
    [早期公開] 公開日: 2015/07/07
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    電子付録
    Chitin oligosaccharide deacetylase (COD) is an enzyme that generates β-N-acetyl-D-glucosaminyl-(1,4)-D-glucosamine from N,N′-diacetylchitobiose. COD has been found only in species of Vibrio bacteria. However, a homology search in the sequence databases revealed that Shewanella woodyi ATCC51908 also encodes a protein with sequence similarity to COD. Analysis of the deduced amino acid sequence of the S. woodyi COD (Sw-COD) confirmed that the protein contains the same motifs as CODs from Vibrio bacteria. The COD-encoding gene, which includes a signal sequence, was cloned from the chromosomal DNA of S. woodyi, the expression plasmid containing this gene was constructed, and then the plasmid was introduced into Escherichia coli HMS174(DE3) cells. The recombinant Sw-COD (Sw-rCOD) was produced in culture medium with the aid of the signal peptide and purified from culture supernatant. The properties of Sw-rCOD (substrate specificity, optimal pH, etc.) were similar to those of the CODs from Vibrio bacteria.
  • Takahiro Noda, Shigenobu Takigawa, Chie Matsuura-Endo, Koji Ishiguro, ...
    2015 年 62 巻 4 号 p. 159-164
    発行日: 2015年
    公開日: 2015/11/20
    [早期公開] 公開日: 2015/08/31
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    This work demonstrates the properties of calcium-fortified potato starch prepared by immersion in natural mineral water containing an extremely high level of calcium (468 ppm) and its food application. The calcium content of the fortified potato starch produced by use of the original mineral water was as high as 813 ppm, while calcium content of the control potato starch was 99 ppm. Rapid visco-analyzer data revealed that the calcium-fortified potato starch had a markedly lower peak viscosity and breakdown and a higher peak viscosity temperature than the control potato starch. Furthermore, calcium fortification caused a significant decrease in starch swelling power. Pound cakes made from the calcium-fortified potato starch and wheat flour blends tended to have a higher specific volume and sensory score of appearance than those made from the control potato starch and wheat flour blends. These findings suggest that the use of calcium-fortified potato starch is critical for making pound cakes with good quality in appearance.
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