The transplantation of cultured bone cells is expected to become a candidate for bone regeneration therapy. For the clinical application of this therapy, there remain several problems to be overcome, for example, the improvements of scaffolds and culture techniques. In this review article, two kinds of porous ceramics, a novel sintered porous hydroxyapatite and a porous beta-tricalcium phosphate (TCP) will be introduced.. The two ceramic scaffolds proved to be applicable for bone regeneration therapy.. Moreover, for the application of this therapy to the regeneration of large bone defects, we improved the culture method by applying a low-pressure system and a perfusion system. Both culture systems accelerated the formation of bone in vivo in this transplantation model. The novel technique for three dimensional cartilage regeneration using RWV bioreactor was introduced. Combinations of the scaffolds and culture techniques might be considered when designing therapeutic strategies.
A thin-film coating of hexamethyldisiloxane (HMDSO) and an O2-plasma treatment were carried out on titanium (Ti) plates. The contact angle of HMDSO-coatings without O2-plasma treatment against double-distilled water was more than 100 degrees, and dramatically decreased after the O2-plasma treatment with less than 5 degrees, resulting superhydrophilicity. Cell proliferation and the alkaline-phosphatase activity of osteoblast-like cells increased on the surfaces of HMDSO-coating with O2-plasma treatment compared to on the Ti and HMDSO-coatings without O2-plasma treatment. SEM observation revealed that the cultured cells were spread on O2-plasma modified surfaces. These results indicated that HMDSO-coating with O2-plasma treatment was a promising for fabricating with osteogenesis surface of scaffolds.
If Platelet Rich Plasma (PRP) can guarantee the quantity of 3 times that the normal blood flow in bone regeneration and soft tissue healing process, it is assumed that it approaches the mesenchymal stem cell, and raises the osteogenesis. We removed prominent gutta-percha in the maxillary sinus for part of the root perforation and did the operation of the maxillary sinus floor augmentation. We reports the case that the new bone created by PRP which is extracted making use of the method of concentrating PRP of the necessary quantity and PRP clot while guaranteeing the blood platelet quantity with the filter and the part of root perforation repaired.
The ultimate purpose of basic and clinical research in tissue regeneration is to recover its function, esthetics, and psychological factor. It is believed that the combination of fig 1 is needed depending on its size, location, or target cell. For example, in severe defect pattern all the factors above are needed. Therefore the key for clinical success is to select the most effective protocol in each therapy. Recently, in maxillofacial surgical field, there are three concepts; physical barrier (such as space making or barrier membrane technique), activation of autogenous cell (such as cortification or autogenous graft), and growth factor (such as in PRP, BMP, Emdogain). Those concepts are utilized singularly or utilized as in combination of two or three. Moreover, safety and simplicity is important to apply the concepts in clinical medicine. In this paper, we discuss an easy and effective method to produce and store PRP before the day of the actual operation. We investigated the use of frozen PRP on the activity of platelets. We also investigated the use of collagen gel as a substitute for thrombin, in order to avoid any chance of infection from human produced thrombin. The possibility of harvesting osteogenic mesenchymal cells from the jaw was also investigated.