The simple and easy certain immobilization method for cell-adhesive protein onto titanium using tresyl chloride (2,2,2-trifluoroethanesulfonyl chloride) was developed. Tresyl chloride, which was liquid compound, was directly applied onto titanium at 37°C for 2 days. Then, tresylated titanium was immersed into the cell adhesive protein solution. Cell adhesive protein was immobilized onto titanium. Quartz-crystal microbalance technique and molecular orbital calculation for model compound revealed the effectiveness of tresyl chloride-activated technique. Initial cell attachment on cell adhesive protein immobilized titanium also showed the usefulness of tresyl chloride-activated technique. Tresyl chloride-activated technique will be applied to three-dimensional scaffold such as titanium fiber mesh ed for regenerative medicine.
Chemical and physical stimuli are said to lead cell proliferation and differentiation. We focused on capsaicin which is considered as a nociceptive stimulus, and examined the expression of capsaicin receptor (TRPV1) in cultured rat bone marrow stromal cells (RBMSCs). RBMSCs were cultured in DMEM with or without capsaicin. The expression of TRPV1 mRNA was examined. TRPV1 mRNA was only expressed in the experimental group. TRPV1 is a nonselective cation channel, can be activated not only by capsaicin but also by heat, acid and lipids. For years, it is said that the expression of TRPV1 was restricted to sensory neurons and CNS. While a number of recent studies demonstrated TRPV1 in a variety of non-neuronal tissues, there are no studies that demonstrated TRPV1 in the bone marrow cells. The present study suggests capsaicin leads the expression of TRPV1 in RBMSCs
Recently, diabetic mellitus is dramatically increasing in our country. A diabetic often induces the serious periodontal disease and following tooth lost. Therefore, there is a necessity for considering the cell activity to the implant materials for the diabetic mellitus. In the present study, we examined the initial cell attachment to titanium plate by using the human gingival fibrobrasts derived from diabetic mellitus patients. The influence of type I collagen coating on titanium plate for the enhancement of initial cell attachment was evaluated. As a result, collagen coating to titanium plate was effective for the improvement of initial cell attachment for non-diabetic mellitus. On the contrary, for human gingival cell derived from diabetic mellitus under the high blood glucose condition of HbA1c 9%, collagen coating to titanium plate was not effective for the enhancement of initial cell attachment.