Basic fibroblast growth factor (FGF-2) could promote proliferation of bone marrow derived stromal cells (BMSCs) and adipose derived-stromal cells (ASCs) in vitro. FGF-2 was reported to have contrasting effects on osteogenic differentiation of ASCs/BMSCs by inhibiting osteogenesis related gene expression in vitro. In this study, we examined and evaluated effects of FGF-2 on cell proliferation, osteogenic relative gene expression, and mineralization of mouse ASC/BMSC culture at different time-points. The osteogenic potential of FGF-2 on cultured BMSCs and ASCs when the growth factor was present in the culture medium for 3 days at different time points or for 7 days was different. The results of this study indicated that FGF-2 supplementation in the culture medium at as late as day 3 resulted in more mineralization in BMSCs, but a lesser effect of FGF-2 on osteoblastic differentiation of ASCs was noted.
A bone defect was created at the center of a rat calvaria. Autologous bone and dentinal granules with autologous bone were transplanted. All specimens were evaluated microradiographically and histologically. CT revealed no difference in the amount of hard tissue between the autologous bone and dentinal granules with autologous bone group 4, 6, and 8 weeks post-surgery. Tissue analysis of the dentinal granules with autologous bone group, 8 weeks post-surgery, revealed that the transplanted autologous bone granules were entirely replaced with the new bone. Meanwhile, about a quarter of the added dentinal granules remained as dentinal granules with autologous bone, and the rest were replaced with autologous bone. In addition, the diameter of the dentin granules decreased with time. Therefore, the addition of dentinal granules to autogenous bone grafts does not inhibit replacement of the new bone and imparts bone regeneration ability to autologous bone grafts for a prolonged period of time.
The purpose of the present study was to investigate the effect of MatriStem derived from porcine bladder on reparative dentin formation and pulp inflammation in vivo compared with mineral trioxide aggregate and calcium hydroxide that have been used for vital pulp therapy. Exposed pulp tissue in the maxilla first molar of 8-week-old Wistar rats was covered with those materials. The rats were sacrificed 4 weeks after the operation. We performed histological examination of hematoxylin and eosin-stained sections of teeth that underwent direct pulp capping, followed by morphometric analysis of formed reparative dentin and evaluation of pulp inflammation. At 4 weeks, MatriStem formed highly compact, intensively reparative dentin with dentinal tubules. MatriStem induced less pulp inflammation than calcium hydroxide. This study suggests that MatriStem is a biocompatible, biodegradable, and bioactive material with growth factors such as bone morphogenetic proteins for dentin regeneration.
We herein report on the usefulness of short implants in the posterior mandibular region of a 51-year-old woman with chief complaint of untreated caries of the lower second molar on the left side. Implant surgery was performed using standard Astra Tech protocol. We determined the final drill diameter according to bone quality, and a 4.0×10-mm (diameter×length) implant was inserted into the left mandibular molar region. After 3 months of submerged healing, provisional, screw-retained, reinforced, acrylic restorations rigidly joining the implant were placed on the abutment. The occlusal surface was adjusted to be in slight contact with the opposite dentition. Four months after the placement of the provisional prosthesis, the implant was manually tested for stability, and a definitive metal–ceramic restoration rigidly joining the implant with occlusal surface in metal was embedded on the titanium- based abutment. There have been no serious problems for more than 5 years.