Several researchers have reported that the fuel, lubricant oil, engine type, and driving cycle enhanced the thermal characteristics and reactivity of carbonaceous particles from automotive exhaust. In this study, thermal evaluation using thermal-optical carbon analyzer is applied to quantify organic carbon (OC) and elemental carbon (EC) in PM2.5 samples. This thermal evaluation sought to obtain the thermal characteristics of carbon components in particulate matter and demonstrated to the standard reference matter generated from diesel engines.