It was known that the vehicle fuel consumption under the mobile air conditioner (A/C) operation is increased in accordance with increase of the specific enthalpy in the test cell. In this study, we evaluated the estimation method for the fuel consumption under the A/C operating condition in the representative summer condition based on the relationship between the specific enthalpy in the test cell and fuel consumption. It was found that the difference between the estimated fuel consumption and the measured fuel consumption in the environmental condition of 71 kJ/kg(DA) was approximately 1.6%.