動脈硬化
Online ISSN : 2185-8284
Print ISSN : 0386-2682
ISSN-L : 0386-2682
15 巻, 5 号
選択された号の論文の28件中1~28を表示しています
  • 中村 治雄
    1987 年 15 巻 5 号 p. 1071-1075
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
  • 寺本 民生, 木下 誠, 加藤 泰一, 堀江 行雄, 渡辺 毅, 岡 博
    1987 年 15 巻 5 号 p. 1077-1080
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    We characterized the lipoproteins produced by perfused rat liver in recirculating system. The catabolic rate of the perfusate VLDL in vivo was faster than that of the LDL. The electrophoretic mobilities of both lipoproteins on agarose gel were at alpha mobility, though addition of serum to them resulted in the beta mobility. These observations suggest that VLDL and LDL are produced by the liver independently and that these lipoproteins were secreted as negatively charged.
    Cholesterol feeding resulted in an increased hepatic production of VLDL by several folds but a decrease in the production of LDL. The distribution of radioactivity among VLDL apolipoproteins was not affected by cholesterol feeding. On the other hand, incorporation of radioactivity into apo B-100 of LDL decreased significantly. Hepatic synthesis of apo B, which was estimated by the immunoprecipitable radioactive apo B in the liver, was decreased significantly by cholesterol feeding. This is the first report of the down regulation of apo B synthesis by cholesterol feeding. The decrease in apo B could be the reason of the fatty liver observed in the cholesterol feeding.
  • 中西 俊明, 西田 敏郎, 田原 大悟, 三宅 清兵衛, 長瀧 重信
    1987 年 15 巻 5 号 p. 1081-1088
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    In this report, we describe the interaction of human lipid transfer protein with various lipoproteins and the effects of apoproteins on the lipid transfer protein reaction.
    1) To determine the binding of LTP with isolated lipoproteins, lipoprotein-coupled Sepharose 4B column was used. From the elution profiles of the column, LTP bound to HDL but hardly bound to LDL in the condition of ionic strength 0.16 NaCl-phophate buffer (pH 7.4) at 4°C. The bound LTP was easily dissociated by changing the buffer to low ionic strength medium. The binding capacity of LDL was increased by applying LTP at higher pH or by acylation of the amino group of LDL. These data indicate that both ionic interaction and hydrophobic interaction participate in the binding of LTP with lipoproteins.
    2) To evaluate the effects of apoproteins in the lipid transfer process, we used purified human LTP prepared with our new method and the assay system of proteoliposomes (or liposomes) to LDL. Apo A-I had not only stabilizing effect but also enhancing effect on LTP reaction. Apo A-II or apo E alone had no significant effect on LTP but had some activating effect in the presence of apo A-I. On the other hand, apo D showed some inhibitory effect on LTP reaction.
    These data suggest that the lipid transfer reaction in the plasma is regulated by various modification of lipoproteins and the level of apoproteins.
  • 多田 紀夫, 平田 文彦, 中村 治雄
    1987 年 15 巻 5 号 p. 1089-1096
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    We have isolated lipoprotein particle containingapolipoprotein A-I (apo A-I) directly from human plasma by anti-apo A-I monoclonal antibody conjugated affinity column (apo column A-I).
    Monoclonal antibody against apo A-I was obtained from ascites of BALB/c mouse induced by injection of hybridoma producing anti-apo A-I antibody.
    The lipoprotein particle containing apo A-I (apo A-I particle) was richer in apo A-I and triglyceride (TG) than HDL separated by ultracentrifugation. The mean diameter of apo A-I particle was slightly larger (137±28Å, Mean±S. D.) and more polydisperse in diameter than of HDL (133±22Å). The recovery of these particle protein from the same plasma through two different methods, ultracentrifugation and apo column A-I, was similar.
    Unknown protein of molecular weight about 75, 000, which is not normaly seen in HDL, was detected in apo A-I particle on SDS-polyacryl-amide gel.
    In the study of fat tolerance test, apolipoprotein and lipid composition of both apo A-I particle and HDL did not show any change during fat loading per os, eventhough TG rich lipoprotein (chylomicron and VLDL) was increased extremely in plasma. As chylomicron contains apo A-I, no appearance of chylomicron derived apolipoprotein (e. g. apo B48) in apo A-I particle during fat loading suggested large particle like chylomicron may not be retained by this affinity column.
  • 日比野 彰, 湯川 進, 前田 孝夫, 野本 拓
    1987 年 15 巻 5 号 p. 1097-1100
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    It is well known that low density lipoproteins (LDL) modified in vitro is mainly metabolised in Scavenger cells such as macrophage (Mφ) which could be developed to foam cell. However, there have been no reports concerning the metabolism of LDL modified in vivo in Mφ.
    Thus, the present study was undertaken to clarify whether the modified LDL is catabolized in human monocyte-delived Mφ in vitro.
    The LDL was obtained from hemodialysis (HD) patients who have usually high concentration of serum malondialdehyde (MDA) compared with those of normal controls. In addition, the reduction of the patients MDA was due to the administration of a-Tocopherol (TOC) at a dose of 600mg per day.
    The Binding, incorporation and degradation of LDL were carried out according to the methods of Goldstein and Brown.
    MDA levels in LDL were significant higher in HD-patients than controls. TOC-treatment slightly reduced LDL-MDA levels in HD-patients.
    In binding and uptake, there were no significant differences between patients and controls. On the other hand, there was the significant increase of degradation in patients and the change was slightly recovered toward the degradation of controls.
    In conclusion, it was suggested that MDA-rich LDL obtained from in vivo as well as artificial MDA-rich LDL (in vitro) may receive the accelation of degradation in M φ.
  • 原 豊, 水野 學, 大井 洋之, 八杉 忠男
    1987 年 15 巻 5 号 p. 1101-1107
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
  • ―成年(20歳)健康調査と家族性高脂血症の家族調査より―
    馬渕 宏
    1987 年 15 巻 5 号 p. 1109-1113
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    Normal ranges of serum cholesterol and triglyceride were settled by the data on a population study of 20-year-old persons and a family study of familial hyperlipidemias.
    1) Normal range (mean±2 SD) of serum cholesterol was 102 to 236mg/dl in the study of 20-year-old persons.
    2) Normal range of serum cholesterol was 127 to 231mg/dl in the family study of patients with familial hypercholesterolemia.
    3) Normal range of serum triglyceride was 50 to 170mg/dl in the family study of patients with primary hypertriglyceridemia.
    Thus, normal ranges of serum cholesterol and triglyceride should be 100 to 230 and 50 to 170mg/dl, respectively.
  • ―望ましい血清総コレステロール値について―
    小西 正光, 飯田 稔, 内藤 義彦, 寺尾 敦史, 木山 昌彦, 児島 三郎, 嶋本 喬, 土井 光徳, 小町 喜男
    1987 年 15 巻 5 号 p. 1115-1123
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    We have already reported from our epidemiological studies that serum total cholesterol is a negative risk factor of cerebral hemorrhage and is a positive risk factor of coronary heart disease.
    In this study, we tried to find the optimal serum total cholesterol level preventing both cerebral hemorrhage and coronary heart disease.
    To find the lower limit of optimal serum total cholesterol level, we saw the relationship between serum total cholesterol level and cerebral hemorrhage incidence in our Akita cohort. As the result, the incidence of cerebral hemorrhage was markedly higher in those whose serum total cholesterol was less than 140mg/dl compared with those whose cholesterol levels were higher than that.
    On the other hand, we also saw the relationship between serum total cholesterol and coronary heart disease in our Osaka cohort of employees in order to find the upper limit of optimal serum total cholesterol level. From this study, we have found that the incidence of coronary heart disease was markedly higher in those whose serum total cholesterol levels were more than 200mg/dl than in those of cholesterol level less than that.
    From these results, we conclude that the optimal level of serum total cholesterol is 140-200mg/dl for the prevention of both cerebral hemorrhage and coronary heart disease.
  • 中村 〓久, 矢野 智彦, 小堀 祥三
    1987 年 15 巻 5 号 p. 1125-1130
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    Multiple regression analysis of 16 risk factors, including serum apolipoproteins in angiographically measured coronary stenosis, was carried out in 291 consecutive patients (190 males and 101 females).
    The risk factors were age, total cholesterol (TC), triglycerides (TG), high density lipoprotein-choles-terol (HDL-C), HDL-C/TC, apolipoprotein A-I, A-II, B, C-II, C-III, E, B/A-I, smoking, weight index (WI), glucose tolerance (GT), and blood pressure (BP).
    Severity of coronary atherosclerosis was scored as the extent of disease seen at arteriography (coronary score: CS).
    The order of importance of risk factors to CS in the five groups of subjects studied were as follows, (1) All subjects: TC>smoking>A-I*gt;age>GT, (2) Male group: TC<A-I>smoking>age, (3) Female group: smoking>age, (4) Young group (age below 54 years): smoking<B/A-I>BP, and (5) Old group (age over 55 years): TC>smoking>A-I>GT.
  • 山村 卓
    1987 年 15 巻 5 号 p. 1131-1135
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
  • 秦 葭哉, 中島 久実子
    1987 年 15 巻 5 号 p. 1137-1144
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
  • 伊藤 博夫, 林 洋, 内藤 周幸
    1987 年 15 巻 5 号 p. 1145-1148
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    The relationship of low density lipoprotein (LDL) receptor activity to plasma lipids and apolipoproteins was examined in 12 heterozygous familial hypercholesterolemia (FH) and i n 4 non- FH type II hyperlipoproteinemia. Among the factors studied, each of plasma high density lipoprotein cholesterol (HDL-C), apolipoprotein A-I, C-II and C-III levels showed a significant possitive correlation with the LDL receptor activity. A significant inverse correlation was observed between the LDL receptor activity and plasma LDL-C, apolipoprotein B, apolipoprotein B/A-I ratio and B/A-II ratio, respectively. Where-as, neither plasma total cholesterol, triglyceride, phospholipid, apolipoprotein A-II nor E levels showed any significant correlation with the LDL receptor activity.
    Then we performed a multiple regression analysis, taking the LDL receptor activity as a criterion variable and plasma lipids and apolipoproteins as explanatory variables. Among many explanatory variables, plasma apolipoprotein B and C-II levels were the main determinant factors in the equation of multiple regression. In conclusion, the measurement of plasma apolipoprotein B and C-II concentration may be useful to predict the LDL receptor activity in patient with type II hyperlipoproteinemia.
  • Masahiro ITO, Yoshihisa KAWASE, Kazuko SHICHIJO, Ichiro SEKINE, Masano ...
    1987 年 15 巻 5 号 p. 1149-1151
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    The purpose of this study is to evaluate the morphological changes of the aorta in monosodium-lglutamate (MSG)-treated rats by light and electron microscopy. Thirty seven male MSG rats and thirty one male Wistar rats were used. The animals were sacrificed at the ages of 6, 11, 22, and 56 weeks. The blood pressure of the MSG rats was negatively correlated to the Lee index, and they showed an average 11 to 26 mmHg lower systolic blood pressure than the Wistar rats. Wall thickness, outer diameter, and cross-sectional area were significantly smaller in MSG rats than in Wistar rats at all ages. However, there was no specific trend in media/radius ratio. The incidence of edematous intimal thickening and the volume percent of . medial extracellular matrix were much lower in MSG rats. It is concluded that MSG obese rats have lower blood pressure and less atherosclerotic aorta than Wistar rats.
  • 増田 弘毅, 所沢 剛
    1987 年 15 巻 5 号 p. 1153-1157
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    Various morphological changes have been reported in the blood flow loaded canine carotid arteries by Masuda et al. Acute blood flow load for about one week induced changes in the endothelial cells and in the subendothelial layer. On the other hand, chronic blood flow load induced the structural changes in the deeper layer of the arterial wall, such as the increase of the internal radius and the increase of the elastic fiber in the media. In the present experiments, we focused on the internal elastic lamina (TEL) in the flow loaded artery. Experiments : To induce blood flow change, arterio-venous anastomosis was constructed between the right common carotid artery and the right external jugular vein in 27 adult female beagle dogs. Left common carotid artery was sutured with left external jugular vein in their adventitias and used for sham-operated control. Three dogs were used for non-operative control. Animals were kept for 1 week, 4 weeks and 6 to 9 months. The blood flow ratio (BFR) of the right (shunted) common carotid artery to the left (control) common carotid artery at the final measurement before sacrifice and the blood flow increase ratio (BFIR) of the same artery (blood flow rate at the final measurement/blood flow rate before anastomosis) were used for the indexes of the blood flow loadance. After the final measurement, artery was normotensively fixed (about 100 mmHg) with 3% glutaraldehyde solution and observed histologically and with electron microscope, especially using special stain (tannic acid uranyl acetate) for elastic fiber. Results: In the blood flow loaded shunted arteries (BFR<1.5, BFIR<3.0), small sized longitudinal elastic fibers appeared in the abluminal side of the TEL in 1 week. Duplication and/or multiplication and splitting of the TEL were observed in 4 weeks. The duplication and/or multiplication and the splitting of the IEL was less frequent in 6 to 9 months than those in 4 weeks. Discussion: Kamiya and Togawa showed that the shear stress dependent adaptive dilatation of the canine carotid artery in the same arterio-venous shunted system as this experiments. It is suggested that the changes of the TEL are dependent on the wall shear stress loadance, because wall shear stress is high in 1 to 4 weeks and may be reduced to the control level in 6 to 9 months.
  • 宮田 康司, 古賀 俊逸, 井林 博
    1987 年 15 巻 5 号 p. 1159-1162
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    The relationship of serum apolipoprotein A-IV (apo A-IV) levels and abnormalities in renal function tests in patients with diabetes mellitus was investigated. Serum levels of apo A-IV were measured by rocket immunoelectrophoresis. Serum levels of apo A-IV as well as total cholesterol (TC) and triglyceride (TG) in 36 diabetic patients were higher than those of 50 normolipidemic healthy controls. In diabetic patients, apo A-IV levels were significantly higher in those patients with abnormal BUN, Cr, CCr values or with proteinuria than those without signs of nephropathy. Correlation coefficients between apo A-IV and BUN, Cr and CCr were 0.53, 0.56 and-0.75, respectively. But the levels of apo A-IV were not related to the values of TC, TG and PSP-test.
    The conclusions are as follow: 1) serum levels of apo A-IV are high in diabetic patients with nephropathy. 2) The apo A-IV levels are correlated with the severity of their renal disease.
  • 清島 満, 鳥沢 英紀, 森 厚, 吉田 洋, 山田 昌夫, 武藤 泰敏
    1987 年 15 巻 5 号 p. 1163-1165
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    Serum apo A-IV concentrations were determined in 77 patients with various types of hyperlipidemia and 34 healthy controls by means of SRID. The mean value of apo A-IV in hyperlipidemic patients was 126plusmn;42.0U/dl which was not significantly high as compared with that in controls (116±28.8U/dl). However, apo A-IV level in hyperlipidemic patients with azotemia (BUN≥20mg/dl) was significantly higher than that in those without azotemia (p7lt;0.05) and that in controls (p<0.01).
    In hyperlipidemic patients with azotemia, there were significant correlations were between apo A-IV and BUN (r=0.594, p<0.01) or serum creatinine (r=0.626, p<0.01) levels, although no significant correlation was observed between apo A-IV and serum lipids (TC, TG, and HDL-C) or other apolipoproteins (apo A-I, A-II, B, C-II, C-III, and E) levels.
    In view of the significant correlation between apo A-IV and BUN or serum creatinine levels, we suggest that apo A-IV levels are regulated by the degree of impairment in renal function. Therefore, it is necessary to give full consideration to evaluate the serum apo A-IV levels clinically, particularly in the case of which level shows high, because of above reason.
  • 中井 継彦, 林信 太, 玉井 利孝, 高井 博正, 高橋 貞夫, 佐部 裕幸, 前田 肇, 宮保 進
    1987 年 15 巻 5 号 p. 1167-1171
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    We have reported that the monosodium glutamate (MSG)-induced obese rats develop hypertriglyceridemia due to increment of VLDL. In order to clarify the mechanisms of hypertriglyceridemia in MSG-treated rats, triglyceride secretion rates (TGSR) and postheparin lipolytic activities (PHLA) were studied. MSG (4mg/g body weight/day) was injected subcutaneously to the neonatal Wistar rats for 5 days. Experiments were performed using the rats fasted for 14 hours, 24 weeks after MSG injection. TGSR was calculated from the differences in plasma triglyceride concentrations before and 90 minutes after intravenous injection of Triton WR 1339 (135 mg/rat). Postheparin lipolytic activities were measured in plasma obtained from the rats, 3 minutes after injection of heparin, 50 U/250 g body weight. Higher Lee's indices were observed in the MSG-treated rats. Plasma concentrations of triglycerides were 361.5±50.8mg/dl (control 100.4±12.8mg/dl) and 883.2±180.6mg/dl (control 72.9±12.0mg/dl) in male and female MSG rats, respectively. TGSR was significantly increased in the MSG-treated rats (male: MSG 495.1±71.2, control 233.8±12.8mg/dl/hr, p<0.005; female rats: MSG 672.5±22.5, control 399.7±29.8mg/dl/hr, p<0.001). TGSR showed the positive correlation to plasma concentrations of triglycerides (male rats: r=0.75, p<0.005; female rats: r=0.77, p<0.005). Significantly increased levels of plasma IRI (male rats: 15.13±2.16 vs. 6.08±0.35, μU/ml, p<0.005; female rats: 9.53±1.20 vs. 4.42±0.32μU/ml, p<0.005) correlated positively to TGSR. There were no significant differences in total PHLA. However, increased activities of lipoprotein lipase (5.22±0.23, vs. 3.75±0.36μmol/ml/hr) and decreased activities of hepatic triglyceride lipase (10.16±0.26 vs. 11.12±0.22μmol/ml/hr) were observed in the male MSG-treated rats. The data presented indicated that increased TGSR induced by hyperinsulinemia plays an important role in hypertriglyceridemia observed in the MSG-induced obese rats.
  • Masaaki ETO, Kiyoshi WATANABE, Norihide CHONAN, Yasunori IWNSXIMN, Kan ...
    1987 年 15 巻 5 号 p. 1173-1177
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    The aim of this study is to elucidate whether or not plasma β-thromboglobulin (β-TG), an indicator of in vivo platelet activation and release reaction, is related to diabetic vascular complications, state of metabolic control and plasma lipids. β-TG was measured using Amersham RIA kit. Sixty-one diabetic patients were studied. Plasma β-TG levels were elevated in diabetics (86±8ng/ml). There was no significant correlation between plasma β-TG levels and the age, duration of diabetes, fasting plasma glucose levels, hemoglobin A1, and plasma lipid levels. In addition, plasma β-TG levels did not differ among the different types of treatment or types of diabetes or sex.
    No significant difference was noted in plasma β-TG levels between diabetics with and without retinopathy. The diabetics with nephropathy had higher levels of plasma β-TG than those without nephropathy. The diabetics with macroangiopathy showed a decreased tendency for plasma β-TG values as compared with those without macroangiopathy. However, these differences did not reach statistical significance.
    It is concluded that diabetics had higher levels of plasma β-TG, suggesting abnormal platelet function, i. e. its hyperaggregability in diabetes, but that plasma β-TG levels are not related to diabetic vascular complications, metabolic control and plasma lipid levels. Further studies will be required to clarify the significance and cause of its higher levels in diabetes mellitus.
  • ―Superose6ゲルろ過カラム使用にて―
    高橋 貞夫, 玉井 利孝, 高井 博正, 林 信太, 前田 肇, 佐部 裕幸, 中井 継彦, 宮保 進
    1987 年 15 巻 5 号 p. 1179-1183
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    Human lipoproteins were separated on Superose 6 HR 10/30 gel-filtration column using Fast Protein Liquid Chromatography System (FPLC, Pharmacia Fine Chemicals). We used the mobile phase which was composed of 0.15 M sodium chloride, 0.01% EDTA-2Na, 0.02% sodium azide, pH 7.2. The P-500 pump, fraction collector and recorder were controlled by the gradient programmer. A constant-elution flow-rate of 0.75ml/min was achieved and fractions of 0.5ml were collected. Five lipoprotein fractions such as VLDL (d<1.006g/ml), LDL (1.006<d<1.063g/ml), HDL (1.063<d<1.210g/ml), HDL2 (1.063<d<1.125g/ml) and HDL3 (1.125<d<1.210g/ml) and total lipoprotein (d<1.210g/ml) were analyzed. The absorbance of the eluate was monitored at 280 nm using the UV-1 monitor. Concentration of cholesterol, triglyceride and phospholipid in each fraction collected were analyzed by the enzymatic methods and protein concentration was measured according to the method of Lowry et al. Total lipoprotein (d<1.210g/ml) was separated to three peaks. Elution volumes of peak 1, 2 and 3 were 7.7ml, 11.6ml and 15.6ml, respectively. Elution volumes of VLDL, LDL, HDL, HDL2 and HDL3 showed 7.6ml, 11.6ml, 15.5ml, 15.0ml and 15.5ml, respectively. Percent composition of lipids and protein in peak 1, 2 and 3 were comparable to those of VLDL, LDL and HDL fractionated by ultracentrifugation, respectively. Recovery and reproducibility of this method were quite satisfactory. In the present study, a new agarose gel matrix, Superose 6 was used. This gel reduced the separation time and improved the separation of plasma lipoproteins. FPLC system will be a useful method for analysis of plasma lipoproteins.
  • ―全国剖検例を用いた統計学的研究―
    張 念中, 河合 祥雄, 岡田 了三
    1987 年 15 巻 5 号 p. 1185-1191
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    Mental stress from one's occupational circumstances is considered an important risk factor in the occurrence of myocardial infarction. However, because of difficulty in quantitative evaluation of such stress, this issue has not been fully studied. In the present work, we investigated the relationship between deaths from acute myocardial infarction (AMI) and occupation using data from the series of the Annual of the Pathological Autopsy Cases in Japan, in which autopsy cases throughout Japan are described. With regard to the 56, 268 persons who died at the age of 15 or older in 1973, 1974 and 1975, i. e., the year of economic depression due to the first oil shock and the years before and after the oil shock, we compared (I) the mortalities from AMI and frequencies of such deaths among all autopsy cases in Japan in relation to the occupational group in the three years and (II) the mortalities from AMI according to the occupation among 3, 241 randomly selected deaths in the three years. The mortality from AMI for 1973, 1974 and 1975 was 2.6%, 3.7% and 3.0%, respectively, showing a significantly higher value for 1974 (p<0.001). Among persons who died from AMI, the proportion of mental workers, particularly executives and managers, and unemployed/retired individuals was significantly higher (p<0.05) in 1974. As for randomly selected autopsy cases, the mortalities from AMI among mental workers in 1973, 1974 and 1975 were 7.8%, 17.0% and 6.2%, respectively, and the corresponding rates among physical workers were 2.0 %, 1.8% and 2.6%. Thus, the mortality among mental workers was significantly increased in 1974 (p<0.05), whereas there were no such variations among physical workers. In addition to the corroboration with the previous experience that deaths from AMI are more frequent among mental workers, particularly executives and managers, than among physical workers, we noted an increase in moratlity from AMI in the period of economic depression. It seems that stress derived from occupational circumstances rather than limited physical activities is involved in the occurrence of AMI.
  • ―とくに血清脂質との関連性について―
    市場 康子, 織部 安裕, 桑原 寛, 高木 良三郎
    1987 年 15 巻 5 号 p. 1193-1196
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    Serum sialic acid and lipid concentration weremeasured in 17 healthy controls, in 13 hyperlipidemic subjects, and in 42 diabetic subjects. In diabetic subjects there was a significant increase in age, fasting plasma glucose, total cholesterol (TC), triglyceride (TG), and LDL-cholesterol (LDL-ch) as compared with those in healthy controls. But there was no significant difference inserum sialic acid and HDL-cholesterol. In female diabetic subjects a significant positive correlation was observed between sialic acid andTC, TG.
    In female diabetic subjects with hyperlipidemia there was significant increase in sialic acid, TC and LDL-ch than healthy controls, hyperlipidemia and diabetic subjects without hyperlipidemia and a significant positive correlation between sialic acid and TG in type IIb hyperlipidemia.
    From these observasions we suggest the correlation between sialic acid and lipid concentrasion in diabetic subjects.
  • 公文 義雄, 吉田 健三, 末廣 正, 大野 文俊
    1987 年 15 巻 5 号 p. 1197-1206
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    Serum amyloid A protein (SAA) was prepared and purified by gel filtration from high density lipoprotein (HDL) obtained from the pooled plasma of patients with bronchiectasis and rheumatoid arthritis. Rabbits were repeatedly immunized with SAA, and the resulting antiserum IgG was rendered monospecific to apo SAA by absorption with normal human HDL and VLDL. Immunoblot analysis, using this monospecific anti-SAA, demonstrated the presence of apo SAA in HDL fractions obtained from the patients with acute myocardial infarction. Two-dimensional gel electrophoresis demonstrated the presence of 6 apo SAA isoforms with a molecular weight of approximately 12, 000, and two major isoelectric points of 6.3 and 5.9. Apo SAA in HDL fractions was determined during the course of acute myocaldial infarction. The apo SAA/apo A-I ratio increased sharply and peaked on the 3rd and 5th days after onset, with 0.90±0.58 (up to 1.68), and declined to an almost normal level on the 10th day. On the other hand, the decrease in plasma apo A-I/HDL-C and apo A-II/HDL-C ratio on the 3rd and 5th days was observed. And during the course, the apo SAA/apo A-I ratio in HDL correlated inversely with the plasma apo A-I/HDL-C and apo A-II/HDL-C ratio (r=-0.538; p<0.001, r=-0.566; p<0.001). In view of these results, the possibility that apo SAA as a constituent of HDL particles might actually change places with apo A-I and apo A-II was suggested. We conclude that these apo SAA-enriched HDL particles, i. e., a remodeled apolipoprotein structure, not only yield abnormal HDL metabolism but also may affect the metabolism of the other lipoproteins.
  • 池田 裕
    1987 年 15 巻 5 号 p. 1207-1211
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    The profile of the distribution of apo E into lipoproteins (apo E profile) had been acquired by author's method that immune complex of apo E was stained after agarose gel-immunofixation electrophoresis using anti-apo E serum. And it had been revealed that apo E distributed in pre-β, β lipoprotein and apo E-containing high-density lipoprotein (HDL-with apo E) which migrated to near the α2-globulin region between pre-β lipoprotein and α lipoprotein, and apo E in HDL-with apo E determined by this method had been named “α2-apo E”.
    In this paper, lipids, apolipoproteins and apo E profile were determined in 10 normal full-term female infants and 10 healthy female adults, in order to characterize the distribution of apo E in umbilical cord serum. The whole serum apolipoprotein levels in mg/dl±S. D. for all neonates were; apo A-I=84.4±11.8; apo B=14.0±4.2; apo E=3.7±1.2. These values represented 62.5% and 15.9% of adult serum values for apo A-I and apo B, respectively, with normal adult values for apo E. The umbilical values of total cholesterol and cholesterol in HDL (HDL-C) represented 31.7% and 81.2% of adult values, and HDL-C represented 60% of total cholesterol in cord serum. The umbilical activity of LCAT represented 26.7% of adult activity, however the cholesterol esterification ratio (esterified cholesterol×100/total cholesterol, %) did not differ between cord serum and adult serum.
    Apo E profile in umbilical cord serum was characterized by the distribution of the majority of apo E to HDL-with apo E, so α2-apo E concentration and α2-apo E ratio (α2-apo E×100/total apo E, %) were significantly high levels of 3.3±1.2mg/dl and 90.6±4.5% in cord serum, in contrast to the levels of 2.0±0.4mg/dl and 50.1±8.0% in adult serum. And in cord serum, α2-apo E was significantly correlated with total cholesterol (r=0.720, p<0.01) and with HDL-C (r=0.688, p<0.01), and α2-apo E ratio reversely correlated with apo B (r=-0.660, p<0.05). So these data suggested the important relationship between α2-apo E and cholesterol metabolism in neonates whose apo B-containing lipoproteins that must transport cholesterol to tissues decreased, in consideration of Innerarity's report suggested that neonates might depend (at least in part) upon HDL-with apo E to deliver to tissues via LDL-receptor.
    Then, umbilical apo E profile was similar to apo E profiles acquired from patients with acute hepatitis or severe hyperthyroidism. But the reduction of HDL-C and reverse correlation between α2-apo E and HDL-C in acute hepatitis were not recognized in cord serum, and umbilical levels of thyroxine and free thyroxine were in normal adult levels. In conclusion, umbilical cord serum was characteristic of the superiority of HDL to apo B-containing lipoproteins in its content, especially apo E distributed for the most part in HDL, being reflected in reduction of apo B-containing lipoproteins.
  • 林 信太, 玉井 利孝, 高井 博正, 高橋 貞夫, 佐部 裕幸, 藤原 隆一, 久津見 恭典, 中井 継彦, 宮保 進
    1987 年 15 巻 5 号 p. 1213-1216
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    The administration of monosodium glutamate (MSG) to a mouse or rat in the neonatal period induced destructive lesions in the arcuate nuclei of the hypothalamus and resulted in a syndrome of obesity, stunting and an abnormal reproductive function. In the present study, plasma lipoprotein metabolism and lipid content in liver were investigated in MSG-treated rats and controls. The MSG treated rats revealed a marked increase of plasma triglyceride concentration and a slight but significant increase of total cholesterol and phospholipids concentrations in both male and female rats. Al-P and T-Bil were also significantly increased in MSG treated rats. There was no significant difference in GOT, GPT and LDH between MSG-treated rats and controls. Triglyceride content in liver was significantly increased in MSG rats than in controls (male:58.9±29.9 vs. 12.5±6.6mg/g of wet weight of liver, female:25.8±8.7 vs. 7.7±2.0mg/g of wet weight of liver). Total cholesterol content in liver was significantly increased in female MSG rats than in female controls. However, total cholesterol content in liver was not different in male rats. There was no significant difference in phospholipids content in liver between MSG-treated rats and controls. In male MSG-treated rats, there were positive correlations between liver function tests and plasma lipid concentrations. We reported hyperlipidemia and increased level of VLDL in MSG-treated rats. These results suggested that the increment of VLDL was related to the abnormalities of lipid contents in liver of male MSG-treated rats.
  • 三神 美和, 本間 康彦, 周 顕徳, 野本 晴夫, 中谷 矩章, 荒木 五郎, 五島 雄一郎
    1987 年 15 巻 5 号 p. 1217-1224
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    The changes in the chemical components of subfractionated lipoproteins were estimated after plasmapheresis up to 28 days. The effects of probucol, cholestyramine, CS-514 on the chemical compositions of lipoploteins after plasmapheresis were also investigated.
    Six FH patients and one non-familial type IIb hyperlipidemic subject were treated. Two to three litters of plasmas were treated at each plasmapheresis by double filtration or dextran-sulfate cellurose column method. Probucol (1g/day), cholestyramine (12g/day) or CS-514 (10-40mg/day) was administered after plasmapheresis.
    TG increased in every lipoprotein fractions immediately after plasmapheresis. VLDL and IDL became cholesterol-rich and tryglyceride-poor molecules gradually without medication. The chemical compositions of LDL1 and LDL2 were relativery constant throughout 28 days after plasmapheresis. HDL2 became triglyceride-rich and apo A-I-rich, and HDL3 became apo A-I-poor immediately after plasmapheresis and return to the same chemical compositions as those of preplasmapheresis. Probucol prevented cholesterol increase in VLDL and IDL. Apo B in LDL1 and LDL2 increased under probucol treatment. HDL2 became cholesterol-rich and apo A-I poor molecules under probucol treatment. Probucol increased apo A-I in HDL3. Cholestyramine had almost no effects on the changes in chemical compositions of plasma lipoproteins after plasmapheresis. CS-514 prevented VLDL-cholesterol increase but had almost no effects on the changes in chemical compositions of other lipoprotein fractions after plasmapheresis.
  • 細川 博之, 奈須 守正, 植嶋 玲子, 末廣 謙, 垣下 榮三, 永井 清保
    1987 年 15 巻 5 号 p. 1225-1229
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    To examine the platelet aggregating activity in a physiological condition, we studied the spontaneous platelet aggregation in whole blood without adding platelet aggregating agents. The spontaneous aggregating rate was determined by the drop in the number of single platelets counted with a Coulter Counter.
    The spontaneous aggregation of normal platelets was increased in proportion to the increment in the stirring rate. We examined the aggregating rate 30 minutes after starting stirring at 500rpm, as the reproducibility was rather satisfactory at this condition. An increment in the aggregation was found in the existence of red blood cells (RBC) (than the condition without RBC.) The inhibition effects to platelet aggregation of dilazep possessing the stabilizing activity of RBC membranes was stronger than that of aspirin. These results suggest that the presence of RBC considerably affects those reactions. In order to remove the effect of ADP from RBC, creatine phosphate and creatine phosphokinase (CP/CPK) were added into this reaction. However this did not inhibit this reaction completely. When both CP/CPK and aspirin were added together, the reaction was not completely inhibited. This suggests the existence of different aggregation mechanism than ADP and thromboxane A2.
    This aggregating rate was significantly higher in patient with DM compared with the control group. These results indicate that the spontaneous platelet aggregating rate in whole blood is valuable for determining platelet hyperfunction.
  • ―アポ蛋白A-I含有Particles, アポ蛋白B-100含有Particlesの組成分析―
    斉藤 俊光, 笠原 忍, 深町 勇, 海津 鬨男, 足立 正一, 加納 啓司, 蒔田 信也, 荻原 弘正, 吉江 康正, 永井 隆, 多田 ...
    1987 年 15 巻 5 号 p. 1231-1236
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    The components of apo A-I and B-100 particles isolated from human plasma by immuno-affinity columns with monoclonal antibodies were analyzed and characterized by using the procedures already described. The apoprotein compositions of apo A-I particles were apo A-I 77%, apo A-II 20%, apo C-II 1%, apo C-III 1%, and apo E 1%. The lipid compositions of apo A-I particles were phospholipid 54%, cholesterol ester 27%, free cholesterol 11%, triglyceride 8%. The component of apo A-I was quite similar to that of HDL. The apoprotein compositions of apo B-100 particles were apo B-100 98% and the mixture of apo C-II and apo C-III 2%. The lipid compositions of apo B-100 were phospholipid 26%, cholesterol ester 28%, free cholesterol 17%, and triglyceride 29%. The component of apo B-100 particles was almost identical to those of VLDL and LDL fractions. It is possible to measure using these affinity columns the amonts of cholesterol and proteins in apo A-I and apo B-100 particles.
  • 高井 博正, 玉井 利孝, 藤原 隆一, 林 信太, 久津見 恭典, 中井 継彦, 宮保 進
    1987 年 15 巻 5 号 p. 1237-1242
    発行日: 1987/10/01
    公開日: 2011/09/21
    ジャーナル フリー
    The effects of oral fat ingestion on plasma concentrations of lipids, lipoproteins and apolipoproteins were studied in fasted 8 healthy men aged 25-43 years (mean age: 30 years old). Any one of cream 200g (49% fat), butter 100g (81% fat) or margarine 100g (81.6% fat) was ingested within 10 minutes early in the morning on three different days. Blood was withdrawn at 0, 60, 120, 180, 240 and 300 minutes after fat ingestion. Lipoproteins were fractionated by sequential ultracentrifugation. Apolipoproteins were measured by single radial immunodiffusion. Fasting plasma concentrations of lipids and apolipoproteins were as follows: Cholesterol (Ch); 190.9±9.6mg/dl, triglyceride (TG); 104.4±12.8mg/dl, phospholipids (PL); 193.6±5.9mg/dl, HDL-Ch; 64.1±2.9mg/dl, apo A-I; 145.0±2.6mg/dl, apo A-II; 32.2±0.7mg/dl, apo B; 75.3±5.9mg/dl, apo C-II; 3.1±0.3mg/dl, apo C-III; 7.7±0.5mg/dl, apo E; 4.1±0.2mg/dl. After each fat ingestion, plasma TG increased mainly due to the increments of VLDL- and chylomicron (d<1.006g/ml)-TG and IDL (1.006<d<1.019g/ml)-TG. There was no significant dif-ference between response of plasma TG after butter load and that of margarine. Plasma Ch increased due to VLDL-Ch and no significant changes of the Ch concentrations in the other lipoprotein fractions were observed. Miyajima et al reported that margarine load for 4 weeks decreased plasma Ch, LDL-Ch and HDL-Ch, while butter load increased LDL-Ch and decreased HDL-Ch. Long term effects of butter or margarine were not reflected in the acute effects of butter or margarine load observed in the present study. Degree of plasma lipids (especially TG) response to fat ingestion varied from subjects to subjects. Increment of plasma TG concentration after fat ingestion correlated significantly to fasting plasma levels of TG: cream (r=0.90, p<0.001), butter (r=0.89, p<0.005) and margarine (r=0.71, p<0.05). The data indicated that degree of TG increase after fat load is partly reflected in the fasting plasma levels of lipids.
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