動脈硬化
Online ISSN : 2185-8284
Print ISSN : 0386-2682
ISSN-L : 0386-2682
21 巻, 8 号
選択された号の論文の9件中1~9を表示しています
  • 嶋田 明彦, 玉井 利孝, 笈田 耕治, 鈴木 仁弥, 高橋 貞夫, 中井 継彦, 宮保 進
    1993 年 21 巻 8 号 p. 529-535
    発行日: 1993/03/01
    公開日: 2011/09/21
    ジャーナル フリー
    Neutral cholesteryl ester hydrolase (NCEH) is an enzyme that mediates the intracellular hydrolysis of cholesteryl ester in cytoplasm. It has been shown to play an important role in the regulation of cholesterol metabolism in the liver. Recently, it has been reported that high-density lipoprotein (HDL)associated cholesterol is processed via a pathway different from that of low-density lipoprotein (LDL) metabolism and that degradation of HDL occurs in a non-lysosomal pathway. In the present study, we examined whether hepatic NCEH activity in H-35 rat hepatoma cells is affected by HDL.
    NCEH activity was increased when the cells were incubated with increasing concentrations of HDL (from 25 to 100μg protein/ml) for 20 hours. Approximately two-fold increases of NCEH activity were obtained with 100μg protein/ml of HDL. NCEH activity was elevated significantly when the cells were incubated with HDL for more than 10 hours. Moreover, NCEH activity was enhanced by dimyristoyl phosphatidylcholine (DMPC)/cholesteryl oleate (CO)/apoHDL compex, as well as native HDL, but not by DMPC/apoHDL or DMPC/CO complexes. In contrast, neither LDL nor VLDL affected NCEH activity. In addition, HDL induced increases in NCEH activity were not inhibited by coincubation with 50μM of chloroquine, which is regarded as an inhibitor of lysosomal hydrolases.
    These findings suggest that uptaken HDL-CE is delivered to a cytoplasmic compartment in a manner distinct from the lysosomal pathway, and is hydrolyzed by NCEH in H-35 rat hepatoma cells.
  • ―住民検診からの成績―
    橋本 隆一, 足達 寿, 鶴田 真, 戸嶋 裕徳, 田代 寛美
    1993 年 21 巻 8 号 p. 537-544
    発行日: 1993/03/01
    公開日: 2011/09/21
    ジャーナル フリー
    Factors related to hypertriglyceridemia were analysed for 168 subjects with impaired glucose tolerance, who were discovered at a screening for diabetes mellitus from a population (n=1219) aged 20 to 69. Hypertriglyceridemia≥150mg/dl was observed in 16.1% (n=27) of subjects. In subjects with hypertriglyceridemia, body mass index, serum cholesterol atherogenic index were significantly higher and HDL-cholesterol was sighificantly lower. Heavy drinking was also a related factor. In parameters from 50g OGTT, the concentrations of insulin at all measurement intervals and the free fatty acids concentrations at 30, 60, 120 minutes postload were higher in subjects with hypertriglyceridemia. Blood glucose concentrations had no statistical significance and results were not affected by adjustments for age and sex. Multiple linear regression analysis (stepforward method) was performed with age and sex as co-variates. The sum of insulin concentrations and free fatty acids at 60 minutes postload were independently related to the concentration of triglycerides.
    In conclusion, hyperinsulinemia and/or insulin resistance and high concentrations of free fatty acids were found to be related to hypertriglyceridemia. These findings might be explained by impaired clearance due to the low activity of lipoprotein lipase and overproduction of triglyceride from free fatty acids.
  • 石田 さくらこ
    1993 年 21 巻 8 号 p. 545-553
    発行日: 1993/03/01
    公開日: 2011/09/21
    ジャーナル フリー
    Urinary microalbumin (UMA) is recognized as a marker of renal complications in IDDM and is associated with increased mortality in NIDDM. We investigated the association of UMA with cardiovascular risk factors in 380 non-diabetic Japanese-Americans. Among 380 of these subjects, 24 (6.3%) were found to have elevated UMA (30-260μg/ml) at the baseline. The subjects with elevated UMA had significantly higher systolic and diastolic blood pressure (BP), triglycerides (TG) and total cholesterol (CH) than those without UMA. Among 355 normoalbuminuric subjects, a 3-year follow-up study indicated that 316 remained at normal levels (NN group) while 39 (11.0%, NM group) developed UMA or proteinuria (260μg/ml∼). Compared to normoalbuminuric subjects, subjects who developed UMA or proteinuria had significantly higher levels of systolic BP, TG, serum glucose, total immunoreactive insulin (ΣIRI) levels during 75g OGTT, and ischemic ECG abnormalities but lower HDL-CH levels at baseline. Only 4.2% of the subjects without hypertriglyceridemia, hyperinsulinemia, high BP and glucose intolerance developed UMA during the 3-year follow-up period. The incidence of UMA was found to increase in accordance with the number of these factors present, reaching 50% in subjects having all four factors.
    To confirm whether UMA is a new potent marker of cardiovascular risk, we investigated the clinical data of these 355 normoalbuminuric subjects for a 6-year period preceding the baseline. The subjects who developed UMA were found to have higher serum glucose, BP, TG and insulin levels but lower HDL-CH levels than those who remained normoalbuminuric, even in the 6-year period before the baseline.
    UMA, which is associated with hypertriglyceridemia, hyperinsulinemia, high BP and glucose intolerance, may thus be a potent marker of increased cardiovascular risk in non-diabetic Japanese-Americans.
  • ―負荷脂肪の脂肪酸の種類の影響と血清脂質, アポ蛋白値との関連―
    坂根 浩弥
    1993 年 21 巻 8 号 p. 555-562
    発行日: 1993/03/01
    公開日: 2011/09/21
    ジャーナル フリー
    Chylomicron is important not only for HDL metabolism but also for the metabolism of apoprotein B containing lipoproteins. Differences in the composition of dietary fatty acids must affect chylomicron metabolism. This study examined the effects of dietary saturated fatty acids, especially palmitic acid, linoleic acid, and oleic acid on chylomicron turnover, and the effects of chylomicron turnover on levels of plasma lipids and apoproteins.
    Fifty-gram doses of creams derived from cow milk (palmitic acid-rich cream, PRC), safflower oil (linoleic acid-rich cream, LRC), and olive oil (oleic acid-rich cream, ORC) were administered with 90, 000 units of vitamin A consecutively at 2 or 3 days interval to 20 subjects. Olive oil only was administered to another 17 subjects. Blood samples were taken before, and 2, 4, and 6 hours after administration of oral cream. Plasma retinyl palmitate (RP) concentrations were measured as an indicator of chylomicron metabolism, using the HPLC method. Absorption-time constants (Ka) and eliminationtime constants (Ke) for plasma RP were calculated by one compartment model analysis.
    Ka was 0.53±0.50/h for PRC, 0.82±1.44/h for LRC, and 1.30±1.93/h for ORC. Ke was 0.31±0.21/h for PRC, 0.31±0.19/h for LRC, and 0.48±0.17/h for ORC. Ka and Ke for ORC were significantly higher than those of PRC and LRC (P<0.05). Ka and Ke values for the 3 different cream types correlated positively with each other (N=20). Olive oil cream was therefore thought to be most appropriate for the oral-fat load test. Ka had positive correlations with plasma HDL-cholesterol (P<0.01), apoprotein AI (P<0.05), and apoprotein AII levels (P<0.01). Ke had also positive correlations with plasma HDL-cholesterol (P<0.01), apoprotein AI (P<0.05), and apoprotein AII levels (P<0.01), and had a negative correlation with plasma apoprotein B concentration (P<0.05). Those results indicated that rapid turnover of chylomicron increased the plasma HDL level and decreased levels of apoprotein B containing lipoproteins. Ke values were significantly low in type II and type IV hyperlipoproteinemias, which probably indicates that chylomicron interfered with uptake of apoprotein B containing lipoproteins via the LDL receptors.
  • 岡田 知雄, 佐藤 良行, 岩田 京子, 岩田 富士彦, 原 光彦, 金 英哲, 渕上 佐智子, 渕上 達夫, 原田 研介, 大国 真彦, ...
    1993 年 21 巻 8 号 p. 563-566
    発行日: 1993/03/01
    公開日: 2011/09/21
    ジャーナル フリー
    The relation of obesity to systolic blood pressure, HDL-cholesterol and ratio of low-and very-low-density lipoprotein cholesterol to high-density lipoprotein cholesterol (atherogenic index: AI) was investigated in 1, 216 subjects aged 9-10, and 12-13 years in Susono City, Shizuoka prefecture. In boys, the risk-factor variables showed strong correlation to trunk fat levels (subscapular skinfold). Among girls, fat levels in the both trunk and limb showed less correlation with the variables than among boys.
    Furthermore, among obese (i.e., obesity index of over 20%) boys, trunk fat levels correlated even more strongly with the risk-factor variables. Since obesity correlates with risk-factor variables in children, prevention of obesity onset in early life may be important in reducing the risk of atherosclerotic disease in later life.
  • ―血清脂質および動脈硬化指数との関係―
    新 啓一郎, 美並 真由美, 高木 正雄, 石山 明寛, 渡辺 文督, 竹中 克, 小俣 政男
    1993 年 21 巻 8 号 p. 567-571
    発行日: 1993/03/01
    公開日: 2011/09/21
    ジャーナル フリー
    Measurement of opening velocity (OV) of the collapsed brachial artery has been suggested as a new noninvasive method of evaluating arteriosclerosis. We therefore examined the relationship between OV and either serum lipids or atherogenic index (AI) in 145 subjects (95 men and 50 women).
    Repeated measurements of OV in one day suggested that OV values for individual subjects remained constant. OV was negatively correlated with age (r=-0.49, p<0.01). Although a highly significant correlation between OV and AT or serum lipids was not observed in all subjects, there were negative correlations between OV and AT or serum triglyceride level in subjects aged 60-79 years. In order to examine the effect of AT level on OV, we divided subjects into a high AT (AI≥3.2) group (H, n=56) and normal AI (AI≤2.8) group (N, n=71) based on AT levels from at least 2 separate measurements. OV was lower in the H group than in N (0.52±0.14 vs 0.59±0.12m/sec, p<0.01). Also, the regression line for OV reduction with age had a steeper slope for group H than N (p<0.05).
    The results suggest tnat OV measurement is a useful noninvasive method of evaluating arteriosclerosis.
  • 松原 三八夫, 渥美 剛, 浪岡 勝彦
    1993 年 21 巻 8 号 p. 573-577
    発行日: 1993/03/01
    公開日: 2011/09/21
    ジャーナル フリー
    Hypothyroidism (Hypo) has been clinically associated with accelerated and premature coronary atherosclerosis. At the same time, lipoprotein (a) [Lp (a)] is known to provoke atherosclerosis and has been confirmed as a major independent risk factor for cardiovascular and cerebrovascular diseases. In this study we examined the role of plasma Lp (a) levels in patients with Hypo.
    Plasma Lp (a) levels in 2 men and 21 women aged 25 to 67 (mean 49) years with untreated Hypo ranged from 2.0 to 58.4 (group A: mean±SE; 19.2±3.0) mg/dl. Levels in 34 healthy men and 72 healthy women aged 30 to 75 (mean 50) years ranged from 0.2 to 46.1 (group B: 11.8±0.8) mg/dl, and those of 2 men and 15 women aged 21 to 77 (mean 58) years with Hypo undergoing treatment for at least 1 year ranged from 1.6 to 47.1 (group C: 13.9±3.2) mg/dl. The mean value for Lp(a) in group A was significantly higher than that of group B (p<0.05), while the value in group C tended to be lower. Following levothyroxine administration to the 17 patients in group A, Lp (a) value for the group declined to 11.9±2.4 (range from 1.5 to 34.6) mg/dl, which followed a similar pattern in group C. In addition, the incidence of Lp (a) levels above 25mg/dl, which is considered highly atherogenic, was higher in group A than in group B (30.4 vs 6.6%, respectively, p<0.001). While 11.8% of group C and 17.6% of group A during treatment had high Lp (a) levels, both tended to be lower than that of group A. A significant correlation was noted between changes of plasma Lp (a) and those of TSH in group A before and during treatment (p<0.05).
    In conclusion, plasma Lp (a) was increased in patients with Hypo, and tended to be lower during treatment. These increased plasma Lp (a) levels may contribute to the increased risk of premature atherosclerosis in the Hypo state.
  • 山田 正道, 山田 哲夫, 早川 富雄, 沢辺 宏, 石黒 友康
    1993 年 21 巻 8 号 p. 579-583
    発行日: 1993/03/01
    公開日: 2011/09/21
    ジャーナル フリー
    The effect of Bezafibrate on early-stage diabetic nephropathy was examined. Twenty-four normotensive non-insulin-dependent diabetes mellitus (NIDDM) patients with persistent microalbuminuria, nineteen of them revealed either hypertriglyceridemia or hypercholesterolemia, were treated with Bezafibrate 400mg/day for six months. Urinary albumin/creatinine ratio (ACR) decreased significantly (P<0.05) from the basal value (73.1±12.2mg/g·Cr; mean±SE) to levels of 79.9±9.3% (1M), 74.8±11.8% (2M) and 64.6±10.5% (6M) during Bezafibrate treatment. In eighteen patients, ACR dropped 25% or more from the basal value. Although a significantly lower serum triglyceride levels, fasting plasma glucose levels and diastolic blood pressure were also observed, a significant association was found only between a reduction of ACR and alterations in serum triglyceride levels; not for the other clinical data.
    These results suggest that treatment with Bezafibrate reduces microalbuminuria in diabetic patients by a mechanism probably linked to changes in lipid metabolism, and could alleviate incipient diabetic nephropathy. Further studies will be needed to assess the anti-proteinuric effect of Bezafibrate.
  • ―歩数計を用いた検討―
    岡村 智教, 飯田 稔, 森山 ゆり, 土井 光徳, 磯 博康, 嶋本 喬, 小町 喜男
    1993 年 21 巻 8 号 p. 585-589
    発行日: 1993/03/01
    公開日: 2011/09/21
    ジャーナル フリー
    To investigate the relation between HDLC levels and walking habits, we performed a cross-sectional study on 459 men aged 40-69 who participated in a health examination of southwest rural towns in 1991-92, and who had not been treated for hyperlipidemia. Before health examinations, estimated paticipants walking habits by using pedometers during three consecutive days. After adjusting for age, body mass index, alcohol intake, and cigarette smoking, average steps per day were positively associated with high-density lipoprotein levels. After the same independent variables were adjusted, multiple regression analysis of HDLC factors indicated that the coefficient for 1, 000 steps per day was 0.7, which was highly significant (P<0.001). These results suggest walking habits are associated with higher HDLC levels and a reduced risk of coronary heart disease.
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