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Volume 23 (1972)
- Issue 6 Pages 295-
- Issue 5 Pages 241-
- Issue 4 Pages 175-
- Issue 3 Pages 123-
- Issue 2 Pages 51-
- Issue 1 Pages 1-
Volume 23, Issue 6
Displaying 1-6 of 6 articles from this issue
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2. Purification of AntibodiesGoro Mogi1972 Volume 23 Issue 6 Pages 295-302
Published: December 10, 1972
Released on J-STAGE: October 20, 2010
JOURNAL FREE ACCESSImmunoglobulins were fractionated from two batches of horse anti-dog thymocyte plasma (ALS). These antiplasmas were made by different immunization schedules, and a considerable disparity was presented in their in vitro activities for cytotoxicity, leukoagglutination, and hemagglutination. One antiplasma showed high titers (high titer-ALS) in these tests while the other showed low titers (low titer-ALS).
Salting out with ammonium sulfate was adopted as a first step for the purification of antibodies. The crude gamma globulin (C.G.G.) obtained by salting out was further fractionated by G-200 Sephadex gel filtration and by DEAE-cellulose column chromatography. The highly purified immunoglobulin G (IgG) was obtained from the 7 S component fractionated by gel filtration. DEAE-cellulose batch production was also used for the isolation of IgG for mass production. The adequate ratio of mixing the wet DEAE-cellulose and C.G.G. solution (1.0g/100ml protein concentration) was 1 to 1.
The cytotoxicity of the low titer-ALS was mainly located in the 7 S component, while that of the high titer-ALS resided in both the 7 S and 19 S fractions. The hemagglutinin activity was mainly in the 19 S fraction in both the high titer and low titer-ALS.View full abstractDownload PDF (2884K) -
its histopathological studyMichiyuki Ariizumi1972 Volume 23 Issue 6 Pages 303-321
Published: December 10, 1972
Released on J-STAGE: October 20, 2010
JOURNAL FREE ACCESSAfter 72 guinea pigs were exposed to sulfur dioxide of four different concentrations (7, 49, 125, 1070ppm), and were also exposed to the mixture of sulfur dioxide and coal-dust in various lengths of time, the pharynx, larynx, trachea and lung were histopathologically studied, and the results were as follows:
1) The pharyngeal mucosa showed no extensive pathologic change even after the animals were exposed to sulfur dioxide of high concentration in a short period of time, but there was thickening of mucosa and cellular infiltration within the submucous layer after they were exposed to sulfur dioxide of low concentration in a long period of time.
2) In the larynx, cellular infiltration in the submucous tissue, edema and other pathologic change occured in the relatively early stage of the experiment, and these changes tended to be prominent in the posterior surface of the epiglottis, glottis and subglottic space
3) In the trachea, detachment and disappearance of ciliated epithelium, cellular infiltration in the submucous tissue, necrosis of epithelium, cellular infiltration within and out of the tracheal cartilage were the noticeble changes.
4) In the lung, the effect of sulfur dioxide was most prominently found, and pulmonary congestion, thickening and cellular infiltration of the interalveolar tissue, localized pulmonary edema and emphysema were recognizable and these changes were more remarkable in the animals exposed to the mixture of sulfur dioxide and coal-dust.View full abstractDownload PDF (10451K) -
its histological change with increasing ageNorio Endo1972 Volume 23 Issue 6 Pages 322-344
Published: December 10, 1972
Released on J-STAGE: October 20, 2010
JOURNAL FREE ACCESSFrom cadavers ranging from newborn to 78 years of age, 122 laryngeal specimens were collected and the epiglottis, ventricular band, glottic space, vocal cords as well as the subglottis, lining epithelium of the arytenoid, tunica propria of mucous membrane, lymphatic structure and submucous tissue were morphologically studied. It was then noted that these tissues showed a systematic pattern of their changes with increasing age. The results were as follows:
1) Epithelial cells of mucous membrane
Ciliated columnar epithelium lining the most part of the larynx except for the vocal cords which were covered by stratified squamous cells was gradually displaced by stratified squamous epithelium with aging process. On the laryngeal surface of the epiglottis stratified squamous epithelium spread from the upper to the lower part, and in the ventricle it spread from the ventricular band and the lateral edge of the vocal cord into the ventricle, and in the subglottis and arytenoid it spread toward the trachea. Over the age of 50 years, stratified squamous and ciliated columnar epithelium became thinner.
2) Tunica propria
It was particulary prominent in the epiglottis and ventricle in newborn but its increase and decrease in amount were related with development and regression of the subepithelial tissue associated with advancing age. This change was not prominent in the subglottis, and tunica propria became increased with associated inflammatory change in the arytenoid area.
3) Lymphatics
The lymphatic system was not well developed in the lingual surface of the epiglottis, and in the laryngeal surface primary lymph nodules and in the arytenoid and ventricle primary and secondary nodules became apparent. The secondary nodules were no more detected over 30 years of age.
4) Glandular tissue
Glands were sparse in newborn but much better developed with growing age up to 19 years. Over 40 years regression of glandular tissue and increase of its surrounding connective tissue took place with increasing age. But in the subglottic area there was no remarkable change in the glandular tissue.
5) Ligaments and muscles ot tne vocal cords
In newborn larynx ligaments and muscles showed paucity of their amount, but in puberty elastic fibers in the ligaments were increased and after second decade the ligaments showed marked changes. Muscle bundle in the vocal cords much more developed with advancing age but after second decade it was changed morphologically and interstitial substance became abundant.View full abstractDownload PDF (8819K) -
Satoshi Kitahara, Tadashi Akitaya, Shigeji Saito1972 Volume 23 Issue 6 Pages 345-350
Published: December 10, 1972
Released on J-STAGE: October 20, 2010
JOURNAL FREE ACCESSIn the precious report*, the authors described how the stenotic larynx was visualized on X-ray films with powdered tantalum. Further studies were performed in patients with laryngeal stenosis and the precious report was confirmed in the facts that many cases of stenotic larynx were only visualized with powdered contrast medium such as tantalum.
The hypopharynx and the cephalic part of the stenotic larynx could be easily visualized wih Dionosil, but the stenotic part and its caudal part could be visualized much successfully with Powdered Tantalum. The cephalic part with Dionosil and the caudal part with Tantalum were contrasted simultaneously.
In most of laryngeal stenosis, tracheotomy was already performed because of difficulty breathing, and the powdered tantalum was sprayed into the trachea through the tracheotomy stoma.
Tantalum laryngography was also useful in follow-up of the patients because of a lach of complication of difficulty breathing.
* J of Japan Bronchoesoph. Scoity Vol 22 No 6View full abstractDownload PDF (3612K) -
Keiji Bono1972 Volume 23 Issue 6 Pages 351-366
Published: December 10, 1972
Released on J-STAGE: October 20, 2010
JOURNAL FREE ACCESSThe present study was performed to establish a method for diagnosis of mild esophagitis by using exfoliative cytology. The exfoliative cytology was made on 25 cases with mild esophagitis and or 20 cases without esophagitis.
The cytological findings of mild esophagitis were compared with that ot normal esophagus, and the characteristics and the chronological change of cytologic smear of esophagitis after being treated locally were reported and shown in photographs. It was revealed that there were rather many cases with mild esophagitis which did not show any clinical local symptom.View full abstractDownload PDF (3937K) -
The Delineation of Foreign Body with Tantalum PowderShigeji Saito, Satoshi Kitahara, Tadahara Ino, Nobuyuki Kokawa, Hirosh ...1972 Volume 23 Issue 6 Pages 367-370
Published: December 10, 1972
Released on J-STAGE: October 20, 2010
JOURNAL FREE ACCESSFor removal of radiopaque foreign body in the air way, the authors has usually used the ventilation bronchoscscope with an aid of fluoroscope and image amplifier. However, success of removal of radiolucent foreign body depends much on surgeons skill, and in case of peanut in the air way the highly skillful technique is required.
Recently, the authors attempted to change a radiolucent peanut to a radiopaque and to remove the peanut under fluoroscope.
A peanut in the air way visualized through a bronchoscope and tantalum powder was sprayed as to delineate the surface of the peanut which became radiopaque and well visualized in an image amplifier under fluoroscope. Every piece of crached peanut could be removed completely and safely.View full abstractDownload PDF (2486K)
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