馬・抗犬リンパ球血清 (ALS) より免疫グロブリンを分離し, in vitroにおける細胞傷害性および凝集性抗体価ならびに赤血球凝集性抗体価をしらべた。ALSは抗原頻回注射法によって得たhigh titer-ALSとtwo pulse methodによるlow titer-ALSの2種類を使用した。
精製の第1段階として硫安塩析法を用い, 全血清より粗精製免疫グロブリン (CGG) を得た。この時の硫安の濃度はALSのin vitro活性および得られた蛋白量からみて0.4飽和が適当であった。
CGGはG-200セファデックス・ゲルろ過法によって7Sおよび19S成分に分けられ, 7S成分はさらにDEAE-セルローズ・カラムクロマトグラフィーによって5つの分画に分離された。またCGGよりDEAE-セルローズ・バッチクロマトグラフィーによってIgGを分離したが, この場合湿潤DEAEセルローズとCGG溶液 (1.0g/100ml蛋白濃度) の混合割合は1: 1が適比であった。
リンパ球傷害性抗体活性はlow titer-ALSではおもに7S成分に認められたが, high titer-ALSでは7Sおよび19Sの両分画にほぼ同程度に認められた。赤血球凝集性抗体はhigh titer-ALSおよびlow titer-ALS共におもに19S成分に存在した。
○本論文の要旨は第23回日本気管食道科学会総会で口演した。
○本研究は米国ワシントン大学医学部耳鼻咽喉科学教室において, NIH Grantsの援助のもとに行なわれた。

抄録全体を表示

After 72 guinea pigs were exposed to sulfur dioxide of four different concentrations (7, 49, 125, 1070ppm), and were also exposed to the mixture of sulfur dioxide and coal-dust in various lengths of time, the pharynx, larynx, trachea and lung were histopathologically studied, and the results were as follows:
1) The pharyngeal mucosa showed no extensive pathologic change even after the animals were exposed to sulfur dioxide of high concentration in a short period of time, but there was thickening of mucosa and cellular infiltration within the submucous layer after they were exposed to sulfur dioxide of low concentration in a long period of time.
2) In the larynx, cellular infiltration in the submucous tissue, edema and other pathologic change occured in the relatively early stage of the experiment, and these changes tended to be prominent in the posterior surface of the epiglottis, glottis and subglottic space
3) In the trachea, detachment and disappearance of ciliated epithelium, cellular infiltration in the submucous tissue, necrosis of epithelium, cellular infiltration within and out of the tracheal cartilage were the noticeble changes.
4) In the lung, the effect of sulfur dioxide was most prominently found, and pulmonary congestion, thickening and cellular infiltration of the interalveolar tissue, localized pulmonary edema and emphysema were recognizable and these changes were more remarkable in the animals exposed to the mixture of sulfur dioxide and coal-dust.

抄録全体を表示

From cadavers ranging from newborn to 78 years of age, 122 laryngeal specimens were collected and the epiglottis, ventricular band, glottic space, vocal cords as well as the subglottis, lining epithelium of the arytenoid, tunica propria of mucous membrane, lymphatic structure and submucous tissue were morphologically studied. It was then noted that these tissues showed a systematic pattern of their changes with increasing age. The results were as follows:
1) Epithelial cells of mucous membrane
Ciliated columnar epithelium lining the most part of the larynx except for the vocal cords which were covered by stratified squamous cells was gradually displaced by stratified squamous epithelium with aging process. On the laryngeal surface of the epiglottis stratified squamous epithelium spread from the upper to the lower part, and in the ventricle it spread from the ventricular band and the lateral edge of the vocal cord into the ventricle, and in the subglottis and arytenoid it spread toward the trachea. Over the age of 50 years, stratified squamous and ciliated columnar epithelium became thinner.
2) Tunica propria
It was particulary prominent in the epiglottis and ventricle in newborn but its increase and decrease in amount were related with development and regression of the subepithelial tissue associated with advancing age. This change was not prominent in the subglottis, and tunica propria became increased with associated inflammatory change in the arytenoid area.
3) Lymphatics
The lymphatic system was not well developed in the lingual surface of the epiglottis, and in the laryngeal surface primary lymph nodules and in the arytenoid and ventricle primary and secondary nodules became apparent. The secondary nodules were no more detected over 30 years of age.
4) Glandular tissue
Glands were sparse in newborn but much better developed with growing age up to 19 years. Over 40 years regression of glandular tissue and increase of its surrounding connective tissue took place with increasing age. But in the subglottic area there was no remarkable change in the glandular tissue.
5) Ligaments and muscles ot tne vocal cords
In newborn larynx ligaments and muscles showed paucity of their amount, but in puberty elastic fibers in the ligaments were increased and after second decade the ligaments showed marked changes. Muscle bundle in the vocal cords much more developed with advancing age but after second decade it was changed morphologically and interstitial substance became abundant.

抄録全体を表示

In the precious report*, the authors described how the stenotic larynx was visualized on X-ray films with powdered tantalum. Further studies were performed in patients with laryngeal stenosis and the precious report was confirmed in the facts that many cases of stenotic larynx were only visualized with powdered contrast medium such as tantalum.
The hypopharynx and the cephalic part of the stenotic larynx could be easily visualized wih Dionosil, but the stenotic part and its caudal part could be visualized much successfully with Powdered Tantalum. The cephalic part with Dionosil and the caudal part with Tantalum were contrasted simultaneously.
In most of laryngeal stenosis, tracheotomy was already performed because of difficulty breathing, and the powdered tantalum was sprayed into the trachea through the tracheotomy stoma.
Tantalum laryngography was also useful in follow-up of the patients because of a lach of complication of difficulty breathing.
* J of Japan Bronchoesoph. Scoity Vol 22 No 6

抄録全体を表示

The present study was performed to establish a method for diagnosis of mild esophagitis by using exfoliative cytology. The exfoliative cytology was made on 25 cases with mild esophagitis and or 20 cases without esophagitis.
The cytological findings of mild esophagitis were compared with that ot normal esophagus, and the characteristics and the chronological change of cytologic smear of esophagitis after being treated locally were reported and shown in photographs. It was revealed that there were rather many cases with mild esophagitis which did not show any clinical local symptom.

抄録全体を表示

For removal of radiopaque foreign body in the air way, the authors has usually used the ventilation bronchoscscope with an aid of fluoroscope and image amplifier. However, success of removal of radiolucent foreign body depends much on surgeons skill, and in case of peanut in the air way the highly skillful technique is required.
Recently, the authors attempted to change a radiolucent peanut to a radiopaque and to remove the peanut under fluoroscope.
A peanut in the air way visualized through a bronchoscope and tantalum powder was sprayed as to delineate the surface of the peanut which became radiopaque and well visualized in an image amplifier under fluoroscope. Every piece of crached peanut could be removed completely and safely.

抄録全体を表示