Pure cultures of each of the five bacterias (Candida albicans, E. coli, Klebsieila, Ps. aeruginosa and Sta. aureus.) were selected as possible sources of contamination and were placed in a separate petri dish. The mixed bacterial growth was prepared using a sample taken from each of the above. This bacterial growth medium was checked by colony count after being cultured for 24 hours using the wire-loop method of 100 and 1000 dilutions.
The F.F.B. which had been sterilized completely by Ethylen Oxide Gas or Formalin Gas, was immerced in the mixed bacterial growth for 15 to 20 minutes.
City water, Benzalkonium chloride (B.C.) 0.1% solution and Bronopol (well balanced electrolytes solution containing 0.1% of glucose) (B.P.) 200μg/ml were used for rapid sterilization of the F.F.B. after being contaminated by the above method.
Conclusive sterilization results of each solution were as follows.
City water cleansing was adequate in several cases but not 100% effective.
For the complete rapid sterilization of the surface of the F.F.B. it was necessary to use B.C. followed by city water and repeat the process twice. When B.P. was used three washings were needed to accomplish the same sterilization process. The scope is then rinsed with water to remove the B.P. from the F.F.B.
The complete rapid sterilization for the channel of the F.F.B., it was necessary to use B.C. (or B.P.), 10cc→City water, 10cc→B.C. (or B.P.), 100cc→City water, 10cc.
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