In regard to melezitose assimilation of industrial yeast, we focused attention on
MAL genes and attempted to elucidate them by a molecular biological approach. When we cultured maltose or melezitose as a carbon source with the brewers yeast except the sake yeast, expression of the
AGT1,
MALx1,
MAL13 and
MALx2 were detected. When we cultured maltose as a carbon source with the sake yeast, the expression of
AGT1 and
MALx2 were detected, but the expression of
MALx1 and
MAL13 were feeble. These sake yeast differed from other yeast as follows:1) the base sequence of
MAL13 differed greatly; 2) chromosome VII did not have
AGT1, and
MAL13 and
MALx1 were adjacent on the chromosome IV; 3) there were few
MALx1 homology arrangements.
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