Caffeic acid ethyl ester (CAEE) was produced during the second fermentation stage of sweet–potato
shochu production. CAEE was produced by the addition of chlorogenic acid (ChA) instead of sweet–potato in the fermentation with koji and yeast. CAEE was not produced, however, during the ethanol fermentation by yeast with a YPD medium with ChA added. It was confirmed that CAEE was produced by incubation with koji, ChA, and ethanol. When the koji was preheated and the enzymes were inactivated before the addition of ChA and ethanol, CAEE was not produced during incubation.
View full abstract