The production of acid protease and acid carboxypeptidase by black and white
koji on a barley medium was tested. At acidic pH and low NaCl concentrations, extracted dried bonito was hydrolyzed by
koji 1.5 month at 45°C. The amount of contaminated bacteria in the
moromi was very low and resulted in a good bonito aroma for the seasoning.
We conducted a sensory test of evaluation by women students for dishes made with the new seasoning. Students felt the seasoning was strong with a salty, acidic and
umami (good) taste.They judged it against available seasonings. Moreover, according to a factor analysis of the seasoning characteristics, it produces a good, strong flavor with traditional Japanese dried bonito stock, and it has characteristic strong tastes with acidity and bitterness. The seasoning received a good overall evaluation.
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