JOURNAL OF THE BREWING SOCIETY OF JAPAN
Online ISSN : 2186-4012
Print ISSN : 0914-7314
Volume 108 , Issue 11
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  • Takashi Watanabe, Haruyuki Iefuji, Hiroko K. Kitamoto
    2013 Volume 108 Issue 11 Pages 823-829
    Published: 2013
    Released: February 13, 2018
    JOURNALS FREE ACCESS
    The purpose of this study is to improve the nitrogen removal ability of wastewater treatment methods using yeasts. In the case of Saccharomyces cerevisiae, we previously confirmed that a positive selection of methylamine-resistant mutants to obtain Ure2 mutants was useful for improving the nitrogen removal ability. Therefore, we tried to improve the nitrogen removal abilities of wastewater treatment yeasts in the same manner and H. anomala IFO0963-HNA36 was obtained. Strain HNA36 removed 1.2-fold higher amounts of nitrogen from rice Shochu distillery wastewater than the wild type strain. We also developed another new breeding method to improve the nitrogen removal ability. A mutant constitutively secreting proteinase, Pseudozyma antarctica GB-4(0)-PNR26 was obtained. Strain PNR26 removed 1.14-fold higher amounts of nitrogen from rice Shochu distillery wastewater than the wild type strain. These results show that nitrogen removal abilities of wastewater treatment yeasts can be improved without recombinant techniques.
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