JOURNAL OF THE BREWING SOCIETY OF JAPAN
Online ISSN : 2186-4012
Print ISSN : 0914-7314
ISSN-L : 0914-7314
Search
Advanced search
OR
Browse
Search
Volume 110, Issue 2
Displaying 1-9 of 9 articles from this issue
Show all abstracts
    • |<
    • <
    • 1
    • >
    • >|
review
research paper
  • Estimates in several industrial yeast strains ploidy by flow cytometer with rapamycin.
    Takeo MIKI, Noboru MURAMATSU, Kazuhiro SOEKAWA, Shinji IKARI, Mikio HA ...
    2015 Volume 110 Issue 2 Pages 105-109
    Published: 2015
    Released on J-STAGE: April 12, 2018
    DOIhttps://doi.org/10.6013/jbrewsocjapan.110.105
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The present work investigated ploidy measurement methods by flow cytometer (FCM) in industrial yeast treated with rapamycin. Four industrial yeast strains such as Saccharomyces cerevisiae OC-2, W-3, KN-35 and KN94, growing in a logarithmic phase with rapamycin, had their ploidy analyzed by FCM. It was apparent that, compared with the case of rapamycin, the untreated peak indicating the DNA amount of the yeast cells as determined by FCM showed increased accuracy over conventional ploidy yeast measurements. Comparison with S. cerevisiae OC-2 and W-3 as diploids estimated the ploidy of industrial yeasts KN-94 strain to be aneuploid or triploid, and the KN-35 strain to be aneuploid or diploid.
    View full abstract
    Download PDF (308K)
  • Induction of apoptosis of human colon carcinoma cells by an extract of cordyceps militaris soaked in sweetpotato shochu
    Cho SHO, Kozue SAKAO, Tomomi ONO, Yoshikazu TAKASE, Kenichi IWAI, De-X ...
    2015 Volume 110 Issue 2 Pages 110-116
    Published: 2015
    Released on J-STAGE: April 12, 2018
    DOIhttps://doi.org/10.6013/jbrewsocjapan.110.110
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Cordyceps militaris, a genus of ascomycete fungai, is usually used as traditional Chinese medicine. We manufactured Cordyceps militaris spirits, named Kinkirishima (SCS), by soaking Cordyceps militaris in sweetpotato shochu. This study aims to clarify the chemopreventive effect of SCS extract by investigating the inhibition of proliferation and induction of apoptosis in human colon carcinoma cells (HCT-116). Based on bioactivity-guided separation, we found that Fraction 1C-III had strongest inhibitory effects on the proliferation of HCT-116 cells. Molecular data showed that Fraction 1C-III induced the apoptosis of HCT-116 as characterized by DNA fragmentation, the activation of caspase-3, and the cleavage of poly (ADP) ribose polymerase (PARP). The major bioactive component is clarified as cordycepin using HPLC, FT-IR, and NMR analysis.
    View full abstract
    Download PDF (324K)
    • |<
    • <
    • 1
    • >
    • >|
feedback
 Top