The acetic acid bacterium NCI 1193, which produces a moderate concentration (12%) of acetic acid, was identified as
Acetobacter pasteurianus from the physiological and biochemical characteristics and DNA analyses of the 16S rDNA and 16S-23S rDNA spacer regions. When the genes encoding alcohol dehydrogenase, aldehyde dehydrogenase, and ubiquinol oxidase were amplified and sequenced, the putative amino acid sequences were homologous to those of the corresponding genes from other acetic acid bacteria. Subunits I and II of alcohol dehydrogenase showed a higher similarity to A.
aceti than A.
pasteurianus, suggesting that NCI 1193 was closely related to
A. aceti.
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