The rats whose median eminence of the hypothalamus was destroyed were used for bioassay of ADH with modified DISCKER'S method. The constant hydration was maintained by the intravenous route and changes in urinary volume were used as the index of response. It was shown that the experimental diabetes insipidus rats were about four times more sensitive to ADH than the normal rats. The smallest dose which could be detected in the present study was 2.5μu per rat. ADH level in normal human plasma was less than 2.1μu/ml after dehydration of 13 to 16 hours.

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This report concerns with the experimental hypertension induced by the repeated injection of dinitrophenol and monoiodoacetate to dog. The author succeeded to get the hypertension in 14 out of 17 cases. The venous return, renal clearance, urinary catecholamine, total 17-OHCS excretion and serum cholesterol were examined.

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There have been already many investigations on the circulatory effects of Valsalva maneuver, and especially with regard to the change of pulmonary circulation it has been made clear that the venous return to right heart during straining is blocked by the increased intrathoracic pressure, and then by the sudden fall of the intrathoracic pressure after release of straining the blood remaining stagnant in the face and extremities suddenly flows into the right heart and the stroke volume of the right ventricle increases compared with that at rest and there is a transient rise of the pulmonary arterial pressure and the net pulmonary capillary pressure. The purpose of this report is to present the electrocardiographic changes by the Valsalva maneuver in patients of chronic broncho-pulmonary diseases in an attempt to detect any specific electrocardiographic changes in pulmonary hypertension or latent cor pulmonale and to discuss their clinical significance. Materials and Methods: (1) One hundred and eleven cases have been chosen for this study, including 76 cases of chronic respiratory diseases, 15 cases of non-cardiopulmonary diseases, 5 cases of neurocirculatory asthenia and 15 cases of healthy adults. The 76 cases of respiratory diseases consists of 35 cases of pulmonary tuberculosis (including 12 cases of after operation and 6 cases of severe pleural involvement), 10 cases of pulmonary emphysema, 8 cases of bronchiectasis, 20 cases of chronic bronchitis and bronchial asthma and 3 cases of lung tumor. (2) First the routine electrocardiogram at rest was recorded, and then, during and following the Valsalva maneuver at lead I, II, III, V₁ and V₅. The Valsalva maneuver was performed by having each subject maintained an expiratory pressure of at least 40mm.Hg for about 10 seconds by blowing into a aneroid sphygmomanometer. Next, of 20 cases including 10 cases taken at random out of the patients of severe broncho-pulmonary diseases and another 10 cases of healthy adults and in-patients without cardiopulmonary diseases, the author recorded their electrocardiogram at rest and following Valsalva maneuver at a speed of 50mm per second, photographically enlarged it, and observed the changes in the ventricular activation time and QRS amplitude at V₁, studying the measured values of both groups in comparison. The respiratory function was judged from the ventilatory function measured with a 9.0 L or 13.5 L Bendict-Roth type respirometer.

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Role of hyperlipemia to the development of arteriosclerosis is commonly recognized from experimental, clinical, etiological and nutritional aspects. Furthermore, by the application of gas chromatography to the fatty acid analysis, abnormality of plasma fatty acid composition has been reported in arteriosclerotic patients and confirmed. The plasma fatty acids are composed of esterified fatty acids which are contained lipoproteins, and albumin bound free fatty acids. And there exists such differences in metabolic pathway between these fatty acids that it seems very important to consider the disorder of fatty acid compositions in arterio-sclerosis from the aspect of plasma lipoproteins. In this study, the fatty acid compositions of ultracentrifugally separated various lipoproteins and albumin bound free fatty acid were determined in the normal and arteriosclerotic subjects and compared between both subjects. Furthermore transport of ingested linoleic acid was studied in the both groups. Method s Subjects and samples: 5 cases of male patients with coronary heart disease and 5 cases of matched control were utilized as subjects. Blood samples were obtained at the overnight fasting state. Thirty gr. of linoleic acids were administrated orally to the each 1 case of both groups of subjects. Then, blood samples were drawn 4 hours after administration. Biochemical procedures: 1) Sf20-10⁵, SfO-20, high density lipoproteins and albumin fraction were fractionated by ultracentrifugation. 2) Various lipoprotein lipids and F. F. A. were separated by the technic of thin layer chromatography. 3) Fatty acid compositions were determined by the technic of gas chromatography. Results 1) Fatty acid compositions of same kind of lipid in various lipoprotein fractions were al-most consistent in the both subjects. 2) In the arteriosclerotic subjects, increase of palmitic and oleic acid percentages and de-crease of linoleic acid percentage were observed in the cholesterol ester and phospholipid fatty acid compositions. However, no changes were observed in the triglyceride fatty acid composition. Changes in fatty acid composition of F.F.A. were almost consistent with those in cholesterol ester and phospholipid. 3) 4 hours after the linoleic acid administration, increase of linoleic acid was observed in the triglyceride of all lipoprotein fractions and extent of increase was most significant in the Sf20-10⁵ lipoprotein triglyceride. Futhermore, degree of increasing extents of linoleic acid were almost consistent between normal and arteriosclerotic subjects. In the F.F.A. increase of linoleic acid was also observed .

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Purpose Renin-Angiotensin system is thought to play the most important role as the pathogenesis of human and experimental renal hypertension. The following studies were performed to clearify some factors of chronic renal hypertension and furthermore the effects of reno-portal venous anastomosis which was shown to be effective to reduce the renal hypertension. Experimental Method Adult mongrel dogs weighing 11 to 12kg were used in this study and were divided into following three groups ; Group I...Dogs with one side kidney (left) was removed and was normotensive (used as control) Group II...Dogs with left kidney removed and were made hypertensive for more than one month by stenosis of right renal artery by mean of metal clipping. Group III...Dogs were made hypertensive as described in Group II and then right renal vein was anastomosed end to side to portal vein. In these three groups of dogs, Angiotensin II, in the dose of 2.5μg/min./kg was infused intravenously for 30 minutes and the changes of B.P. were measured 2 to 5 minutes interval before, during and after the infusion. In Group I and II the urine was collected 20 minutes after the end of Angiotensin II i. v. infusion and Rf values were measured by paper chromatography using the mixture of n-butanol, acetic acid and water solution in the ratio of 4: 1:5 (v/v) as medium. Then, ¹³¹I-Angiotensin II in the dose of 2.5g/min./kg was infused intravenously for 20 minutes after dogs were given Lugol solution. Thenafter, radioactive counts were measured by well-type scintillation counter in 1ml of the arterial blood and the urine obtained soon after the end of infusion and in 1gm Speimens of the brain (brain stem), heart, lung, liver, spleen, pancreas, kidney and calf muscle obtained immediately after dogs were sacrificed and autoradiography was also studied in the same materials as described above except urine, after these samples were homogenized with perchloric acid and centrifuged, separated and supernatant was neutralized by KOH and filtered.

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Frank lead system, one of the corrected orthogonal VCG leads, was applied to 118 normal young men in order to establish standards and to clarify the process of the ventricular activation quantitatively. Materials and methods: The subjects used in this study were 1 18 normal young men, ranging from 1 8 to 30 years. The three planar projections of VCG were photographed simultaneously on 60×60mm X-ray films. Vector loops were interrupted by saw-toothed waves of 680cps, thus indicating the conduction speed and the rotating directions of the QRS loops. The three scalar ECG of FRANK system as well as the conventional 12 leads ECG were recorded with paper speed of 100mm per second. Vector loops were enlarged 6 times using the photographic enlarger and were traced on the section papers. Morphological observations of the vector loops were such as the directions of initial and terminal vectors of the QRS loops and the directions of inscription of QRS and T loops. Analytico-geometrical analysis of vector loops were performed using electric computer "OKITAC 5090, Type H", using orthogonal coordinates of each vectors. Scales for angles were shown in Fig. 1. The normal ranges were determined on the basis of 95 per cent and 98 per cent limits. The items of the quantitative parameters used in this study were as follows: 1 . Directions and magnitudes of maximum QRS and T vectors: Maximum vectors in each plane and the projections of the spatial maximum vectors on each plane were measured. Frontal plane; Magnitude: √(X²+Y²), Angle: tan^-1X/Y Sagittal plane; Magnitude: √(Y²+Z²) Angle: tan^-1Z/Y Horizontal plane : Magnitude : √(Z²+X²) Angle: tan^-1Z/X Spatial magnitude: √(X²+Y²+Z²) 2. QRS-T angles and ratios: QRS-T angles in each plane were defined as algebral difference from the angle of maximum QRS vector to that of maximum T vector. The spatial QRS-T angles (θ) were calculated by the following formula:

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