Multiwavfelength X-ray diffraction studies were performed on cytochrome
c' (Cyt.
c') and superoxide dismutase (SOD) crystals. Multiwavelength intensity data were collected for the crystals using synchrotron radiation produced by the vertical wiggler at the Photon Factory, National Laboratory for High Energy Physics. Positions of two irons in the Cyt.
c' crystal were determined from difference Patterson maps based on anomalous scattering caused by irons. Fourier maps calculated from the multiwavelength data indicated clear molecular boundaries. Positions of the Cu
2+ and Zn
2+ sites in the crystal of SOD were located and distinguished from difference Fourier maps by enhancing anomalous scattering effects of Cu
2+ and/or Zn
2+.
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