Effects of pyruvate, citrate, succinate and glycolate on the incorporation of
15NH
4 and
15NO
3 into amino acids and amides, and on the amino acid contents were studied by using the seedlings of Norin No. 41 of rice. 1. Effect of organic acids on the incorporation of
15N from
15NH
4Cl or Na
15NO
3 into amino acids and amides (Figs. 1 and 2). In the root, glutamic acid in
15NH
4-feeding case and glutamine in
15NO
3-feeding one were the most strongly labelled. The incorporation of
15N into amino acids and amides tested was decreased by the organic acid treatments in both cases of ammonium and nitrate. In the top, glutamine, glutamic acid and aspartic acid were relatively strongly labelled with
15N from both the
15NH
4- and
15NO
3-feedings. The effect of organic acids on the incorporation of
15N into amino acids and amides was decreased in the
155NO
3-feeding, but it was not found in the
15NH
4-feeding. In the endosperm, the incorporation of
15NH
4 into glutamine was increased by the treatment with organic acids. 2. Effect of organic acids on the contents of amino acids and amides (Tables 2, 3, 4 and 5). In the root, the contents of amino acids and amides were increased by the treatment with organic acids in the NH
4-feeding, but not in the NO
3-feeding. In the top, the amounts of aspartic and glutamic acid were increased by the organic acid treatments in the NH
4-feeding, but no the remarkable differences on the contents of amino acids and amides were found in NO
3-feeding. In the endosperm, the effects of organic acid treatments to produce these nitrogenous compounds scarcely were found.
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