口腔衛生学会雑誌

Kasaiの培地を改変したものを使用して長さ1μ大の微小な嫌気性Vibrioの15株を歯垢から得た。記載的研究の結果, この菌が同定される既往の菌種は見付からなかった。この菌の鞭毛は菌体の凹彎側で中央から菌端寄りの場所に数本附着していたので, Selenomonasとは区別すべきであった。嫌気性半月状菌に関する記載の不完全な現状から考えて, この種の菌の鞭毛観察には電子顕微鏡の使用が絶対に必要であると考察された。

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In the recent reseach of topical application of fluoride, Muhler and his coworkers reported that stannous fluoride is more effective than sodium fluoride. In this experiment, the amounts of fluorine or tin in or on protected powdered enamel were determined, which was treated with 2per cent solution of sodium fluoride or 1, 2, 4, 8 or 16 per cent solution of stannous fluoride respectively. The procedure was as follows. Using mechanical shaker, 100mg. of 100-150 mesh enamel powder was treated with sodium fluoride or stannous fluoride solution for 5 minutes at 20°C. respectively, (single application) and in addition, the samples similarly treated four times in repetition were also prepared. For determing fluorine in enamel powder, samples were ashed and steam-distilled, and determined with Zirconium-Alizarin method. Samples protected similarly prepared served also for determination of tin. They were dissolved in 4 N HCl and determined with Cacotherine method. Following conclusions were obtained.
1. Samples treated four times with 2%NaF contained about triple amounts of fluorine as compared with that of singly applied samples.
2. In case of SnF₂ application, singly treated samples contained 50-60 μg of fluorine per 100mg, while four times treated one contained fluorine only 10-20 μg over that in single applications mentioned above.
3. The uptake of tin in samples treated with 1% or 2% SnF₂ were much more than that treated with 8 % or 16 % SnF₂. Such tendency was more marked in case of four times applications.
Considering the uptake of fluorine or tin in enamel together with acid solubility of powdered enamel treated with SnF₂, the amounts of uptake of tin appeared to play a dominant role in reducing the acid solubility of enamel, particularly in the earlier decalcifying stage.

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