巻号一覧
18 巻 (1968)
- 3-4 号 p. 121-
- 1 号 p. 1-
18 巻, 3-4 号
選択された号の論文の7件中1～7を表示しています
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津田 理子1968 年 18 巻 3-4 号 p. 121-132
発行日: 1968年
公開日: 2010/10/27
ジャーナル フリーThe ether extract of incubated saliva (several days incubation in an aerobic condition) contains only one or two compounds, phenol or phenol and indole, as light absorbents in the ultraviolet region. This paper deals with the determination of these compounds.
The optical density of the extract was proportional to the concentration of phenol or indole in the material (Figs. 9 and 10). Each 3 ml of the materials, saliva and standard solutions, was extracted with each 6 ml of ether or petroleum ether. The optical densities (O.D.) of these extracts were estimated at 2872 Å in the case of petroleum ether (P.E.) and at 2730 Å in the case of ether (E.). Procedures were summarized in next column. When the concentration of indole or phenol in the standard solution is 1mg/100 ml water, the content of phenol or indole in saliva can be calculated by the following equations;
Indole (I): a/c mg/100 ml saliva
Phenol (P): b-d·I/e mg/100 ml saliva
In this method the recovery was 99-103% (Table 7) and the relative error 1.9% (Table 8).抄録全体を表示PDF形式でダウンロード (2000K) -
山田 茂, 酒井 洋一, 中久木 正俊, 清水 洋, 青木 宏祐1968 年 18 巻 3-4 号 p. 133-136
発行日: 1968年
公開日: 2010/10/27
ジャーナル フリーWe researched the PMA indices using elementary school children as their subjects, and tried to find the correlation coefficients between anteriors and molars.
The result of investigation was as follows.
The correlation coefficients between anteriors and molars were low such as 0.28-0.32, and those between first molars and premolars were relatively hight such as 0.70-0.73.
Therefore, it is more practical to evaluate the oral health by means of PMA indices of anteriors and first molars than those of the anterior region.抄録全体を表示PDF形式でダウンロード (625K) -
境 脩, 大谷 広明, 千葉 義男, 大西 正男, 鈴木 丈信1968 年 18 巻 3-4 号 p. 137-142
発行日: 1968年
公開日: 2010/10/27
ジャーナル フリー東芝鶴見工場, 本州製紙江戸川工場, 本州製紙岩淵工場の従業員, 計365名を対象として歯周疾患の集団管理を実験的に実施した。東芝, 本州の両事業所でそれぞれ実験群と対照群を設定し, 両方に対して, 1) 歯肉のう, 2) 歯牙沈着物, 3) 欠損歯数の検査を行ない, 実験群に対しては歯石沈着活動度試験の結果に従つた間隔での歯石除去ならびに刷掃指導を行ない, なお東芝実験群にはさらに歯肉切除術を行なつて, 次のような結果を得た。
盲のう陽性者数は東芝, 本州ともに実験群は減少したが, 対照群は増加し, その増加率は大きく, 実験群, 対照群との間には高度の有意差 (p<0.001) をみた。すなわち上記のような集団管理方式は盲のう形成の予防に対して有効であつた。
欠損歯は両事業所とも対照群は実験群に比べて増加していたが, 有意差はなかつた。しかしこの増加率が今後も続くと仮定すれば, 東芝では全実験期間3年4カ月, 本州では2年4カ月で有意差を得ることを推算できた。
歯石沈着活動度試験の結果から, 各実験群の年間必要除石回数の算出ができ, 東芝では平均歯石沈着活動度0.79±0.06mgで, 57人に対して年50回の除石が必要となり, 本州では1.19±0.14mgで, 72人について年90回の除石が必要となつた。
以上のような集団管理方式を歯科衛生士が行なうとしたとき衛生士1人の管理できる人数の算出が可能となつた。東芝例では年約3,200人, 本州例では年約2,600人であつた。なおこの管理可能数は主として集団の平均歯石沈着活動度から推算できると考えている。抄録全体を表示PDF形式でダウンロード (1151K) -
常光 旭, 中村 亮, 渡辺 達夫, 岩本 義史1968 年 18 巻 3-4 号 p. 143-148
発行日: 1968年
公開日: 2010/10/27
ジャーナル フリーPhosphomonoesterase [EC 3.1.3.1, 3.1.3.2; orthophosphoric monoester phosphohydrolase], found in human beings, is widely distributed in the animal, plant, and microorganism kingdoms, and is now generally recognized to have an important role in the metabolism of nucleotides, phospholipids and carbohydrates as well as protein synthesis.
The existence of alkaline and acid phosphatases in human whole saliva is apparent, but their origins and physiological actions in oral field remain to be determined. The purpose of this study was (1) to clarify the existence of phosphomonoesterase in human parotid saliva, (2) to purify the phosphomonoesterase, and (3) to investigate its some properties.
About 500 ml of human parotid saliva were collected into cold bottles from 15 male adults, 20 to 30 years old, by the method of Keene. Then the saliva was fractionated with ammonium sulfate and the fraction precipitating between 30 and 70 per cent was chromatographed on DEAE- cellulose column. Through these procedures, phosphomonoesterase in parotid saliva has been partially purified about 42-fold to a specific activity of 3.33 (μmoles of liberated p-nitrophenol per mg of protein). Using this enzyme sample, some properties were investigated. The optimal pH of this enzyme was 3.3 and the Km for p-nitrophenyl phosphate, α-glycerophosphate and β-glycerophosphate was calculated to be 3.6×10-5M, 2.2×10-3M and 1.0×10-3M, respectively. The maximum velocity for these three substrates was almost equal. This fact shows that this enzyme has no specificity for these substrates. When the enzyme kept in 0.2 M glycine-HCL buffer, pH 3.3 at 50°C, it was more stable than that in 0.2 M TrisHCL buffer, pH 7.0. The half life of this enzyme was about 60 minutes. Additions of SnF2 or NaF inhibited completely their enzyme activities at 1×10-3M concentration. Magnesium chloride, p-CMB had no effect on the enzyme activity.抄録全体を表示PDF形式でダウンロード (1124K) -
高畑 肇, 森岡 俊夫, 松村 敏治1968 年 18 巻 3-4 号 p. 149-154
発行日: 1968年
公開日: 2010/10/27
ジャーナル フリーThe peroxidase antibacterial action in human saliva against species of lactobacilli has been described by Klebanoff and Luebke, Iwamoto et al. Inoue, Saji, Morioka and Matsumura have reported that the antibacterial activity is distributed in certain tissues of rat including parotid, submaxillary, lacrimal, and mammary gland.
In each study, however, this antibacterial activity was detected in SL broth against lactobacilli. Inoue et al. have also reported that the salivary antibacterial (S.A. Factor) activity was obtained in the selected media including Trypticase soy broth by treating with the-SH group reagent, CuSO4.
It is conceivable that the salivary peroxidase antibacterial system plays an important role as a natural defensive function in the oral cavity. In order to clarify the defensive function of the antibacterial system, nonselected culture medium such as Trypticase soy other than SL broth should be used obtaining the wide variety of antibacterial spectrum against oral microorganisms.
As the growth rate of the oral microorganisms in culture media, in general, is low, an estimation of the growth inhibition by the antibacterial system on the microorganisms is hard to quantify, and the viable cell count of the certain microorganisms such as cariogenic streptococci is also hard to carry out.
It is suggested that in these cases the antibacterial activity would be adequately estimated by the cup plate method.
In the present work, experiments were carried out to determine whether the antibacterial activity could demonstrate quantitatively in the non-selective medium such as Trypticase soy.
The salivary peroxidase antibacterial activity was successfully demonstrated in the cup plate method with Trypticase soy agar containing 8.3×10-5M CuSO4. Growth of Lactobacillus plantarum ATCC 8014 was apparently inhibited by the antibacterial system, and the growth inhibition is completely depressed by catalase.
The cup plate method is strongly suggested to be useful for the quantitative estimation of the salivary peroxidase antibacaterial activity against various species of oral microorganisms.抄録全体を表示PDF形式でダウンロード (1287K) -
第1報 学童のう蝕および斑状歯罹患状態上田 喜一, 飯塚 喜一, 藤村 豊, 大橋 和夫, 森 崇, 高江洲 義矩, 近藤 武, 江川 隆通, 樋出 守世, 塚脇 篤太郎, 金平 ...1968 年 18 巻 3-4 号 p. 155-165
発行日: 1968年
公開日: 2010/10/27
ジャーナル フリーA survey of prevalence of dental caries and mottled enamel was made on 1, 200 school children (6-12 years of age) at Kasaoka city of Okayama prefecture in western Japan, where the municipal water supply had been accidentally fluoridated at 1.2-1.3 ppm or more for nine years.
Results were summarized as follows:
1. At the period of survey, the fluoride concentration of the municipal water supply ranged from 0.9 to 1.3 ppm in tap water of several spots In the city; however, it would have ranged from 1.4 to 1.8 or more during the earlier period of the water supply operation, because there were four water source wells which started respectively to operate at different dates.
2. The domestic well water in this district contained also an average of 0.52 (±0.44) ppm of fluoride.
3. The percent of persons with one or more DMF teeth was significantly lower than that of the non-fluoridated group (inhibitory rate: 40%), while the percent of persons with mottled enamel was in average 40% in this group (however, moderate or severe grade cases were only 8.5%).
4. There were no significant difference in the dental caries prevalence between the municipal water group and the domestic water group, while the mottled enamel prevalence was distinctly higher in the former.
5. Several numbers of moderate grade cases of mottled enamel were found even in groups taking fluoride lower than 1.0 ppm and also there were several cases in taking as low as 0.5 ppm.
6. There is likely a correlation between the fluoride concentration of drinking water and urine in the range from 0.5 to 3.2 ppm in water.抄録全体を表示PDF形式でダウンロード (2114K) -
実験う蝕予防の研究V塩沢 武夫1968 年 18 巻 3-4 号 p. 166-175
発行日: 1968年
公開日: 2010/10/27
ジャーナル フリー1. 市販Wistar系rat130匹を用いて, 生後3週間, 約35g前後で, cariogenic dietを投与し, 室温23℃, 湿度50%で90日間飼育し, その成長と, う蝕の発生を検査した。
2. う蝕の検出法は, 大西らが過去数年間行ってきたWistar ratのう蝕実験で得た結果を集め統計的に処理したう蝕罹患度の表現方法にしたがった。
3. casein. 砂糖, 澱粉, 混合塩オリーブ油ビタミン類の一定量を含む混合飼料は, cariogenic dietであることを確認し, 蛋白源として, caseinを等蛋白量の大豆蛋白と置き換えると成長面では劣ったが, う蝕の抑制は促進された。
4. 大豆蛋白をAlcoholで精製分画し, 得られた蛋白質を混合飼料として, 等蛋白量用いたところ, caseinより劣るが, 大豆蛋白と同程度に成長し, 大豆蛋白の効果以上に, う蝕の抑制効果は大きかった。
5. 以上の効果を追求するため, 実験に用いられたratのdentinの化学組成を検索したところ歯質のCa, P量では変化なく, 総N量が, 大豆蛋白投与群で, 増大した。抄録全体を表示PDF形式でダウンロード (1873K)
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