An in situ study was designed to evaluate the effects of sugar substitutes on remineralization potential using an artificial dental caries model in the oral environment. Palatinose, sorbitol, and xylitol were selected as sugar substitutes. An intra-oral device with thin demineralized enamel was fixed on the buccal surface of the first molar of five volunteers (3 males, 2 females; 20-29 years old). They wore the device for 10 days without brushing. During experimental periods, they rinsed with 2 ppm fluoride solution and with three sugar substitute solutions plus 2 ppm fluoride for 3 minutes. The remineralization potential was compared among the fluoride solution and three sugar substitutes. A group that rinsed with a fluoride solution and a demineralized group served as positive and negative control groups. The degree of remineralization was evaluated by the changes in lesion depth and mineral loss values. In the oral environment condition, the lesion depth values increased by approximately 25 //m even in the positive control groups. Except for palatinose, the fluoride and other sugar substitutes groups showed a trend toward demineralization compared with the negative control group. The improvement rate of the lesion depth was in the order of I fluoride, -16.6%; xylitol, -8.6%; sorbitol, - 7.9%; and palatinose, 0%. However, there were no statistically significant differences among any groups when they were compared with the positive control group. From the mineral loss point of view, the trend of demineralization was also similar to the lesion depth; the improvement rate was in the order of: Sorbitol, -23.8%; positive control, -20.8%; xylitol, -20.8%; and palatinose, -5.1%. However, there were no statistically significant differences among any groups when they were compared with the positive control group. Under this in situ study, there were no signs of remineralization at all. To maximize the remineralization potential of saliva by stimulating the secretion rate through sugar substitutes, it was suggested that plaque has to be removed, saliva should react directly with the lesion, and the use of a fluoride dentifrice was recommended.
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