The purpose of this study was to develop a new method for chemical stain control. The removal of acquired pellicle by an active agent and the mechanism of pellicle removal were examined. Hydroxyapatite (HA) discs mounted on a mouthpiece were held in the oral cavity of three healthy volunteers. The in situ pellicles, periodically formed, were dyed with phloxine, the avalues from the Commission Internationale de 1' Eclairage (CIE) L a b uniform color scale were measured with a spectrophotometer, and the amount of protein was determined by ninhydrin assay. Tea-denatured pellicles, formed with tea rinsing, were brushed with solutions containing chemical agents, and the residual pellicles were evaluated. HA powder was treated with diluted saliva solutions for two hours, then various amounts of pyrophosphates were added, and the amounts of adsorbed pyrophosphates and desorbed proteins were determined. The results showed that the a values correlated significantly with the total protein (r = 0.95, p<0.0l). The pellicles grew with time and reached a plateau after about two hours in three volunteers. The amount of residual pellicle (a value) in the sodium pyrophosphate group (Mean ± SD = 0.73 ± 0.35) was significantly lower than that in the control group (6.28 ± 0.28 ', p<0.01), and was the lowest produced by the agents tested. Scanning electron microscope (SEM) images of residual pellicles showed that pellicles were desorbed by brushing with the pyrophosphate solution, and were physically removed with a toothpaste slurry. The isotherm of pyrophosphates on HA was characteristic of Langmuir adsorption, and a linear relationship between adsorbed pyrophosphates and desorbed proteins was demonstrated. Consequently, pyrophosphate was found to be an effective agent for removal of pellicle. The pyrophosphate adsorbs to the HA surface and breaks the ionic bonds between the pellicle and HA. The potential of pyrophosphate as an agent for removal of stained pellicle was indicated by this study.
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