Journal of Equine Science
Online ISSN : 1347-7501
Print ISSN : 1340-3516
ISSN-L : 1340-3516
Volume 22 , Issue 3
Showing 1-2 articles out of 2 articles from the selected issue
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  • Minoru OHTA, Manabu NEMOTO, Koji TSUJIMURA, Takashi KONDO, Tomio MATSU ...
    Type: -Note-
    2011 Volume 22 Issue 3 Pages 53-56
    Published: 2011
    Released: October 25, 2011
    JOURNALS FREE ACCESS
    A PCR assay for the diagnosis of respiratory disease induced by equine herpesvirus type 1 (EHV-1) was performed at the clinical laboratory in the Racehorse Clinic of the Ritto Training Center of the Japan Racing Association from December 2007 to March 2008. The assay was performed without the trouble of contamination throughout the study and its turnaround time was approximately 6 hr. The PCR detection rates of EHV-1 among seroconverted horses were 22.2% for nasal swabs and 33.3% for blood samples. However, EHV-1 DNA was also detected in horses without seroconversion at a low rate. These results indicated that the PCR assay should be used as an adjunct method, but would help to make an early diagnosis of EHV-1 infection.
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  • Rina KIHARA, Yoshinori KASASHIMA, Katsuhiko ARAI, Yasunori MIYAMOTO
    Type: -Note-
    2011 Volume 22 Issue 3 Pages 57-60
    Published: 2011
    Released: October 25, 2011
    JOURNALS FREE ACCESS
    Injury initiates a repair process characterized by influx of fibroblasts and the rapid formation of fibrous scar tissue and subsequent tissue contraction. The response to injury and behavior of the different tendon fibroblast populations, however, has been poorly characterized. We hypothesized that the fibroblasts recovered from tendon with acute injury would exhibit different cell properties relating to adhesion, migration and tensegrity. To test this hypothesis we evaluated the ability of fibroblasts recovered from normal and injured equine superficial digital flexor tendons (SDFTs). The injured tendon-derived cells showed greater contraction of the collagen gel but poorer adhesion to pepsin-digested collagen, and migration over extracellular matrix proteins compared to normal SDFT-derived fibroblasts. Thus, the cells present within the tendon after injury display different behavior related to wound healing.
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