Ten mule foals were used to investigate serum IgG concentration. Blood was collected from mares and foals on the day following parturition (D1), at 48 and 72 hr after birth (D2 and D3), and on D7, D14, D21, D28, D35, D42, and D60. Serum IgG concentration was determined by zinc sulfate turbidity test and was above 400 mg/dl at all time points, except for one foal on D2. Mean values were above 800 mg/dl from D1 to D7 and at D60. The difference between mares and foals at each collection time was significant, except at D1. For all other days, foal IgG values were lower than the mare IgG values. Mean IgG concentrations were lower from D14 to D42 compared with D1 and D60. The results show that the critical period for the mule foal is at D28, which can be a vulnerable window for infections. The variation pattern in IgG values is similar to that in data published for horse and donkey foals. There are no published studies to date on the subject, and the present contribution enables further understanding of the newborn mule foal.

A cross-sectional survey was conducted to determine the seroprevalence and risk factors influencing the presence of Brucella spp. antibodies in donkeys in Yobe south senatorial zone, Nigeria. The study was aimed at determining the importance of Brucella spp. infection in donkeys (Equus asinus). A total of 200 sera samples from of 105 males and 95 female donkeys were collected and screened for brucellosis using the rose bengal plate test (RBPT) and the indirect enzyme-linked immunosorbent assay (iELISA). Data obtained were analyzed to determine associations and risk factors. The analysis revealed that 21.5% and 18.5% were seropositive by RBPT and iELISA respectively, with 22.0% and 20.0% of the male and female donkeys being seropositive by RBPT, and 19.0% and 17.9% of the male and female donkeys being seropositive by iELISA, respectively. There was a statistically significant association between donkey age and positive rate of iELISA for detecting Brucella infection. Though the positive rate was higher for males than females, there was no statistically significant association between sex and location of donkeys and the sensitivities of RBPT and iELISA for detecting Brucella infection. In conclusion, this study indicates that brucellosis exists with high seroprevalence particularly among male and adult donkeys and is of public health significance and economic importance because it can lead to infertility and abortion in the stock.

A nutritional supplement containing salacinol (NSS) was administered to Thoroughbred foals daily beginning 21 days after birth, and clinical signs and intestinal microbiota were analyzed. The average number of days for which foals exhibited a fever between 21 and 110 days after birth was determined. The number of days was significantly reduced, by approximately 1/3, in the NSS group compared with the control group. Furthermore, improved weight gain was observed in the NSS group compared with the control group. By analyzing the intestinal microbiota, it was determined that the ratio of Clostridium cluster XIVa increased after 3 weeks of NSS administration. These results demonstrate that the daily administration of NSS might improve the intestinal environment of neonatal foals and be useful for health.