Transducing phages were sought among temperate phages harbored by coryneform and glutamic acid-producing bacteria. By using a nonlysogenic subline of
Brevibacterium flavum strain 2247, as the phage indicator, twenty-four temperate phages were isolated from strains of coryneform and unidentified glutamic acid-producing bacteria. Phage CP-119 transduced various markers at frequencies of around 10
-7, and the addition of 4mM cAMP to recipient cultures stimulated the transduction. Phage CP-123 transduced the
trp marker at a frequency of approximately 10
-6 when 1.2M MgCl
2 was added to the reaction mixture. Characteristics of these transducing phages and of the lysogeny states of glutamic acid-producing bacteria were discussed.
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