Two distinct types of acid phosphatase (PMase) formation were recognized in submerged culture of
Aspergillus oryzae and
Aspergillus sojae. One of PMase formation was repressible by inorganic phosphate (P
i) in the medium but the other was promoted by increased concentration of P
i in the medium. These PMase formations were influenced by the pH and pH changes of the medium, and pH 4 to 7 seemed to be adequate. Under the non-proliferating condition in which the pre-cultured mycelium was resuspended in a buffer and shaken, the positive PMase formation was seen after a short lag period, but not in the anaerobic condition. The P
i-repressible PMase formation was not suppressed by the addition of NaF, KCN, or 2, 4-dinitrophenol but was by 8-hydroxyquinoline, and P
i-promoting PMase formation was markedly inhibited by KCN, 8-hydroxyquinoline, and 2, 4-dinitrophenol but not by NaF.
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