In two strains of osmophilic yeast Candida placentae, the formation of smooth spherical to ellipsoidal ascospores was found. Accordingly, the present yeast is transferred to the genus Saccharomyces and named Saccharomyces placentae.
Four forms of acid phosphatase, designated as Ia, Ib, IIa, and IIb according to their elution profiles of column chromatography, were isolated from the solid culture of Aspergillus oryzae. Ia and Ib were mainly produced in phosphate-enriched conditions, and conversely IIa and IIb were formed chiefly in phosphate-restricted conditions. The relation of enzymatic properties between Ia and Ib, or IIa and IIb were very similar, but Ia and Ib could be clearly differentiated from ha and lib. pH optima were about pH 4 for Ia and Ib, and about pH 6 for ha and IIb. Fluoride was a potent inhibitor for Ia and Ib, but not for ha and IIb. These enzymes seem to be non-specific acid phosphatases, phosphoserine, phosphothreonine, and phosphoethanolamine being hydrolyzed with IIa and IIb but not with Ia and Ib.
Cellular fatty acid composition of 9 strains of Bacteroides ruminicola, 16 strains of B. oralis, and 6 reference strains including B. splachnicus, B. melaninogenicus var. melaninogenicus, B. melaninogenicus var. intermedius, B. asaccharolyticus, B. fragilis, and B. vulgatus was determined by gas-liquid chromatography. The organisms tested were divided into three major groups based on composition. In the first group, 6 strains of B. ruminicola and 11 strains of B. oralis, B. melaninogenicus var. melaninogenicus, B. melaninogenicus var. intermedius, B. fragilis, and B. vulgatus were included. Anteiso-15:0 was the most abundant acid, and iso-15:0 and/or 3-OH-iso-17:0 were present in large quantity in most strains of this group. In the second group, 2 strains of B. ruminicola, and 1 strain of B. oralis, B. splachnicus, and B. asaccharolyticus were included. Iso-15:0 was the most abundant acid, and other major acids present were anteiso-15:0, 3-OH-iso-17:0 and so on. In the third group, 1 strain of B. ruminicola and 4 strains of B. oralis were included. A rather large amount of 16:0 was present, and other major acids found were anteiso-15:0 and/or iso-15:0.
A specialized transducing phage ρ11spo0F, containing one of the stage 0 sporulation genes, spo0F, of Bacillus subtilis was constructed. Sporulation of spo0F mutant of B. subtilis was restored by lysogenization with ρ11spo0F, indicating that the mutant allele was recessive to the wild type. The physical map of ρ11spo0F genome was constructed by using restriction endonucleases BamHI and SalI and compared with that of ρ11wt genome. The results indicate that the spo0F gene resides on the SalI-B fragment of ρ11spo0F genome. It was also found that the 1.3×106 dalton EcoRI fragment of ρ11spo0F DNA contained the spo0F gene.
Physiological and chemotaxonomic characteristics of Thiobacillus novellus were reexamined under various conditions for growth. This organism oxidized thiosulfate without inhibition from the presence of organic compounds such as glucose or glutamate. Tetrathionate supported the growth of T. novellus in an autotrophic medium. Optimum pH and temperature for growth were 7.0 in heterotrophic conditions and 7.0-7.4 in autotrophic conditions, and 25-30°. These characteristics were different from those so far described in T. novellus, and therefore the description for T. novellus was amended. Quinone system was coenzyme Q10, and DNA base composition was 67.3mol%. T. novellus required biotin in heterotrophic and autotrophic growth. Cellular fatty acids of the organism consisted of saturated straight chain acids of C16:0 and C18:0, mono-unsaturated straight chain acid of C18:1, and cyclopropane acid of C19 as major components.