The Journal of General and Applied Microbiology
Search
OR
Browse
Search
Volume 27 , Issue 2
Showing 1-8 articles out of 8 articles from the selected issue
    • |<
    • <
    • 1
    • >
    • >|
  • TOSHIO HARA, ANDREA AUMAYR, SEINOSUKE UEDA
    Volume 27 (1981) Issue 2 Pages 109-114
    Released: August 18, 2006
    JOURNALS FREE ACCESS
    Genetic transformation of Pseudomonas aeruginosa by chromosomal DNA is reported. Treatment of cells with CaCl2 was necessary to effect this transformation and intermediate cultivation was also essential. The frequency of transformation with intra- and extracellular DNA was very similar. The divalent ions Mg2+ and Ca2+ improved transformation frequency.
    View full abstract
    Download PDF (533K)
  • ANDREA AUMAYR, TOSHIO HARA, SEINOSUKE UEDA
    Volume 27 (1981) Issue 2 Pages 115-123
    Released: August 18, 2006
    JOURNALS FREE ACCESS
    Bacillus natto Asahikawa produces a considerable amount of polyglutamate (PGA) and its γ-glutamyltranspeptidase (γ-GTP) activity is 80 times higher than that of the transformable strain B. subtilis Marburg which cannot produce PGA. The genetic character of high PGA productivity in B. natto Asahikawa was transferred to B. subtilis Marburg by the DNA-mediated transformation. Among transformants that acquired high productivity of stringy substance, 4 strains produced a higher amount of PGA. The activities of γ-GTP from the representative 4 transformants were 20 times higher than the activity of B. subtilis Marburg. These results suggested the presence of correlation between PGA productivity and γ-GTP activity, and the presence of a specific gene(s) that participated in the production of PGA in B. natto.
    View full abstract
    Download PDF (870K)
  • FRANCIS J. CHLAPOWSKI
    Volume 27 (1981) Issue 2 Pages 125-144
    Released: August 18, 2006
    JOURNALS FREE ACCESS
    Cell-free extracts of the soil amoeba Acanthamoeba palestinensis have been found to catalyze the incorporation of choline, L-serine and ethanolamine into phospholipids of intracellular membranes in the presence of Ca2+ and the absence of other exogenous factors. Cell fractions enriched in endoplasmic reticulum and mitochondria contained the highest activity, while plasma membranes demonstrated no activity. Microsomal pellets were used to characterize the enzyme activity. Mg2+ could not replace Ca2+, but did not inhibit Ca2+-dependent activity, while Mn2+ inhibited activity. Choline incorporation was competitively inhibited by L-serine and less effectively by ethanolamine. L-Serine incorporation was competitively inhibited by ethanolamine and inhibited by choline only at low concentrations. Ethanolamine incorporation was competitively inhibited by L-serine, while choline had no inhibitory effects. The specificities of the competitive inhibition of incorporation of each alcoholic amine indicated that more than one enzyme or enzymatic site was involved and that incorporation occurred via a direct base exchange mechanism.
    Inactivation of incorporation enzymes occurred in an exponential fashion during incubation of microsomal membranes at 29° in the presence of Ca2+. However, the measured rates of inactivation could not account for the low levels of incorporation experimentally observed with each of the three alcoholic amines. Quantitative analysis of the turnover of radioactive bases of prelabeled phospholipids predicted ongoing loss of the labeled bases from phospholipids that was not due to exchange, as well as the unavailability of a constantly increasing fraction of the labeled bases for such loss and/or exchange.
    View full abstract
    Download PDF (1790K)
  • RYOJI SAKAUCHI, SADAO HOSHINO
    Volume 27 (1981) Issue 2 Pages 145-155
    Released: August 18, 2006
    JOURNALS FREE ACCESS
    Rumen samples from bloating steers had volatile fatty acids (VFA) concentrations high in acetate and low in propionate so that the acetate/ propionate ratio was consistently higher in bloating animals than in healthy ones. The ruminal pH values in bloated steers were compatible with those in healthy steers. Ammonia concentrations were higher in bloated steers than in healthy ones. There were no essential differences in the rates of gas production in vitro by rumen fluid between bloated and healthy steers. The total count and generic composition of rumen ciliates were similar in all steers and not likely to correlate with bloat. In contrast, although the total counts of bacteria in bloated steers were of the same order as those from healthy steers, the constitution of microflora was greatly affected by the development of bloat: gram-negative cocci (0.3-1.0μm), gram-positive cocci (0.5-1.1μm) and gram-positive small rods {(0.45-0.65μm)×(0.65-1.2μm)} increased, while gram-negative small rods {(0.35-0.7μm)×(0.9-2.0μm)} decreased in bloating steers.
    View full abstract
    Download PDF (1060K)
  • GEORGE CALDES, BENJAMIN PRESCOTT, CHARLES A. THOMAS II, PHILLIP J. BAK ...
    Volume 27 (1981) Issue 2 Pages 157-172
    Released: August 18, 2006
    JOURNALS FREE ACCESS
    A water soluble polysaccharide from Carthamus tinctorius flowers was found to react with antisera specific to Types III and VIII Streptococcus pneumoniae. Intraperitoneal inoculation of mice with the polysaccharide caused the generation of splenic antibody-forming cells which were similar in specificity to those detected following immunization with Type III pneumococcal polysaccharide. Antiserum reactive to Type III pneumococcal polysaccharide was prepared by immunizing rabbits with the C. tinctorius polysaccharide. Chemical analysis of the polysaccharide showed it to consist mainly of xylose, fructose, galactose, glucose, arabinose, rhamnose, and uronic acid.
    View full abstract
    Download PDF (1925K)
  • YOZO MIYAKAWA, TERUYA KOMANO, YOICHI MARUYAMA
    Volume 27 (1981) Issue 2 Pages 173-183
    Released: August 18, 2006
    JOURNALS FREE ACCESS
    Synchronized cells of Bacillus subtilis 168 (Thy His) and its derivative, DNA elongation mutant ts526 were used to study the effects of the inhibition of DNA synthesis on the cell-cycle-specific events. When DNA synthesis of strain 168 was inhibited by thymine deprivation from the beginning of the cell cycle, septum formation and cell division were possible in the ongoing cell cycle but not in the forthcoming one. The results of the upward shift of temperature experiments with ts526 supported the above observations. Fluctuation of penicillin sensitivity and stepwise increase in peptidoglycan synthesis during the cell cycle, which were observed in the presence of DNA synthesis, occurred only in the ongoing cell cycle in the absence of DNA synthesis. We at least suggest that these cell-cycle-specific events are intimately connected with septum formation and cell division, and are not affected by DNA inhibition in the ongoing cell cycle.
    View full abstract
    Download PDF (964K)
  • H. J. POWELL, J. T. MAY
    Volume 27 (1981) Issue 2 Pages 185-193
    Released: August 18, 2006
    JOURNALS FREE ACCESS
    The effect of fatty acids, monoglycerides, diglycerides and triglycerides (present or able to be generated in human milk by lipase activity) on the growth of anaerobic Bifidobacterium bifidum types a and b, Bifidobacterium infantis sp. infantis types a and b, and facultative Escherichia coli and Salmonella typhimurium were determined turbidimetrically. The fatty acids and monoglycerides tested inhibited the growth of the Bifidobacteria while the gram-negative E. coli and S. typhimurium proved more resistant. Triglycerides were not inhibitory to the bacteria tested, with some actually enhancing growth. Monolaurin and 2-monopalmitin proved to be the most potent inhibitors of Bjfidobacteria growth in the concentration ranges used.
    View full abstract
    Download PDF (851K)
  • FEDERICO FEDERICI
    Volume 27 (1981) Issue 2 Pages 195-197
    Released: August 18, 2006
    JOURNALS FREE ACCESS
    Download PDF (242K)
    • |<
    • <
    • 1
    • >
    • >|
feedback
Top