The patterns produced by nine enzymes of ballistosporogenous yeasts and supposedly related yeasts were studied by electrophoresis on polyacrylamide gel. Forty-two strains belonged to the genera
Sporobolomyces,
Sporidiobolus, Aessosporon and
Bullera and twenty-four strains to
Rhodotorula,
Rhodosporidium, Cryptococcus, unidentified ballistosporogenous yeasts, and
Candida edax.
Four
Sporobolomyces salmonicolor strains, two
Sporobolomyces holsaticus strains, two
Sporobolomyces odorus strains, five
Sporidiobolus salmonicolor strains, two
Aessosporon salmonicolor strains, and one strain of
Aessosporon dendrophilum produced similar electrophoretic patterns. Moreover, mating was observed between some of these strains.
Sporobolomyces roseus and
Sporobolomyces shibatanus differed from
Sporobolomyces salmonicolor in their glucose-6-phosphate dehydrogenase (EC 1.1.1.49.), but the patterns of the other enzymes were similar; all three species differed clearly from
Sporobolomyces singularis, Sporobolomyces gracilis, Sporobolomyces puniceus, and
Sporobolomyces antarcticus.
Sporobolomyces albo-rubescens showed a peculiar 6-phosphogluconate dehydrogenase (EC 1.1.1.41.) pattern and was similar to two
Rhodotorula rubra strains in the pattern of their enzymes. Close relationships were also seen between
Sporidiobolus ruinenii and
Rhodotorula graminis, and between
Bullera alba and a strain in
Cryptococcus albidus var.
albidus in the electrophoretic patterns of their enzymes. Four unidentified strains which had lost the ability to produce ballistospores had patterns similar to those of
Bullera alba, Rhodotorula glutinis, Cryptococcus laurentii var.
flavescens, and
Cryptococcus macerans. Three colorless strains, putatively derived from a strain of
Sporobolomyces roseus, showed the same electrophoretic patterns as the strains from which they originated.
View full abstract