The respiratory activities of
Candida rugosa JF-101 and C.
lipolytica No. 6-20 grown on glucose were studied in relation to the
n-alkanes (
n-C
9H
20-
n-C
18H
38). Both yeasts readily oxidized
n-decane,
n-undecane and
n-dodecane. Cells of both yeasts grown on glucose showed a lag in
n-alkane oxidation, whereas the cells grown on
n-alkane did not.
n-Hexadecane was oxidized by cells grown on the same
n-alkane better than by cells grown on
n-decane and glucose. The respiratory action of each yeast on
n-hexadecane tended to be induced and the activity on
n-decane was constitutive. The glucose oxidation rates of the spheroplasts from
C. rugosa and
C. lipolytica were, respectively, 70-80% and 32-34% as high as those of the intact cells and the
n-decane oxidation rates of the spheroplasts were 4-8% and 0% as high as the respective intact cells. Addition of the non-ionic surfactant Tween 80 restored the action of spheroplasts on
n-decane to 10-19% as high as the intact cells, except for the spheroplasts from the glucose grown cells of
C. lipolytica.
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