On the basis of an extended investigation of morphological, biochemical and physiological properties and its phylogenetic affiliation it is proposed to place
Methanomicrobium paynteri (Rivard et al. 1983,
24), validly published in 1984 (Rivard et al. 1984,
25) within a new genus,
Methanolacinia gen. nov. as
Methanolacinia paynteri comb. nov. Its cell envelope consists of a cytoplasmic membrane and a hexagonally arranged S-layer with a lattice constant of the geometrical subunits of 15.3nm, while the periodic structures on the cell envelope of
Methanomicrobium mobile were not characterized. The S-layer contains a glycoprotein subunit with an apparent molecular weight of 155, 000. The polyamine pattern of
Methanolacinia paynteri is comparable to that of
Methanogenium species but differs significantly from that of
Methanomicrobium mobile. Comparative lipid analyses of
Methanomicrobium mobile,
Methanogenium cariaci, Methanospirillum hungatei and
Methanolacinia paynteri reveal marked differences. Furthermore,
Methanolacinia paynteri grows not only on H
2/CO
2 as reported, but also on 2-propanol/CO
2, 2-butanol/CO
2 and cyclopentanol/CO
2.
Together with the low
SAB-value of 0.51, which
Methanolacinia paynteri shares with
Methanomicrobium mobile, these features corroborate the proposed reclassification.
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