In an attempt to elucidate the phylogeny of the C
1-compound-utilizing bacteria, dendrograms were drawn for gram negative obligate and facultative methanol-utilizing bacteria, a methane-utilizing bacterium, and other gram negative bacteria on the basis of their 5S ribosomal RNA sequences published to date. Phylogenetic distances were calculated using
Knuc, a parameter for phylogenetic distance based on the number of nucleotide substitutions reported by Kimura, and
Dnuc, a modified form of
Knuc proposed by Hori and Osawa. The dendrograms were drawn using the unweighted pair-group (UPG) average clustering method. Obligate methanol-utilizing bacteria belong to one cluster of gram negative bacteria, while facultative methanol-utilizers are members of another. There is no close phylogenetic relationship between obligate and facultative methanol-utilizing bacteria.
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