The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
Volume 49, Issue 5
Displaying 1-7 of 7 articles from this issue
Full Papers
  • Gennadi I. Naumov, Elena S. Naumova, Maudy Th. Smith, G. Sybren de Hoo ...
    Article type: Others
    Subject area: Others
    2003 Volume 49 Issue 5 Pages 267-270
    Published: 2003
    Released on J-STAGE: March 18, 2005
    JOURNAL FREE ACCESS
    Sequence analysis of the D1/D2 domain of 26S rDNA was conducted upon seven Arthroascus strains from different geographic localities. The European and Asian species Arthroascus schoenii was documented from the North-American continent and from the Island of Hawaii. We discuss the heterogeneity of the genus Saccharomycopsis sensu Kurtzman and Robnett 1995. On the basis of molecular and genetic data the genus Arthroascus von Arx is reinstated.
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  • Luca Bánszky, Tibor Simonics, Anna Maráz
    Article type: Others
    Subject area: Others
    2003 Volume 49 Issue 5 Pages 271-278
    Published: 2003
    Released on J-STAGE: March 18, 2005
    JOURNAL FREE ACCESS
    Selenate-resistant mutants were obtained from several strains of Schizosaccharomyces pombe. The obtained mutants all belonged to the same genetic complementation group. They were low in sulphate uptake activity and in ATP sulphurylase activity. They grew on medium containing sulphite, thiosulphate, cysteine or glutathione but not methionine as the sole source of sulphur. From these results, the mutants were concluded to carry mutations in the ATP sulphurylase gene. Inability of the mutants to utilize methionine as a sulphur source is rationalized by the absence of the reverse transsulphurylation pathway in this organism; wild type strains must utilize methionine as a sulphur source after it is degraded to give rise to sulphate.
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  • Takeshi Kobayashi, Michika Kajiwara, Mita Wahyuni, Toshihide Kitakado, ...
    Article type: Others
    Subject area: Others
    2003 Volume 49 Issue 5 Pages 279-286
    Published: 2003
    Released on J-STAGE: March 18, 2005
    JOURNAL FREE ACCESS
    Lactic acid bacteria from “terasi” shrimp paste, a highly popular fermented seafood in Indonesia were isolated and characterized. Viable cell counts were 104 to 106 cfu/g on MRS medium. All the isolates were catalase-negative, gram-positive cocci and were able to grow at 15% NaCl. Numerical phenotypic analysis showed that the isolates clustered into one group. However, they could be classified into two types: the Tetragenococcus halophilus group and the T. muriaticus group as revealed by a restriction fragment length polymorphism (RFLP) analysis and sequencing of the 16S rRNA gene. This study is the first to show that both species of Tetragenococcus are distributed in Indonesian fermented foods.
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  • Koei Hamana, Takehiko Tanaka, Ryuichi Hosoya, Masaru Niitsu, Takashi I ...
    Article type: Others
    Subject area: Others
    2003 Volume 49 Issue 5 Pages 287-293
    Published: 2003
    Released on J-STAGE: March 18, 2005
    JOURNAL FREE ACCESS
    Cellular polyamines of newly isolated acidophilic, thermophilic and thermoacidophilic archaebacteria were investigated for the chemotaxonomic significance of polyamine distribution profiles. In addition to spermidine, spermine and agmatine, a quaternary branched penta-amine, N4-bis(aminopropyl)spermidine, was found in thermophilic Thermococcus waiotapuensis, Thermococcus aegaeus and Pyrococcus glycovorans belonging to the order Thermococcales. An acidophilic euryarchaeon, Ferroplasma acidiphilum located in the order Thermoplasmatales, contained spermidine and agmatine. Norspermidine, spermidine, norspermine and spermine were found in thermoacidophilic Acidilobus aceticus and thermophilic Thermodiscus maritimus located in the order Desulfurococcales, and in thermophilic Pyrobaculum arsenaticum, Pyrobaculum oguniense, Vulcanisaeta distributa and Vulcanisaeta souniana belonging to the order Thermoproteales; however, the four genera differ on their tetra- and penta-amine levels. Thermophilic Staphylothermus hellenicus belonging to Desulfurococcales contained caldopentamine, caldohexamine and N1-acetylcaldopentamine in addition to norspermidine, spermidine and norspermine. This is the first report on the occurrence of acetylated penta-amine in nature.
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