A novel Gram-negative, aerobic, rod-shaped and pesticide (chlorpyrifos and atrazine) tolerant strain, designated CW-D 3
T, was isolated from a heavily polluted soil sample in Jiangsu Province, China. The strain could tolerate about 800μg ml
-1 atrazine or chlorpyrifos when added in TYB medium. A polyphasic taxonomy including phylogenetic and phenotypic analysis was performed on the new isolate. Phylogenetic analysis and chemotaxonomic characteristics revealed that strain CW-D 3
T belongs to the genus
Klebsiella. Its closest phylogenetic neighbors were
K. singaporensis (strain LX3
T), three subspecies of
K. pneumoniae (strains ATCC 13883
T, ATCC 13884
T and ATCC 11296
T) and
K. granulomatis, according to 16S rRNA and
rpoB gene analysis. The 16S rRNA gene sequence similarity was about 94.8-97.6% and the
rpoB gene sequence similarity was about 96.8-98.6% to its phylogenetic neighbors. Strain CW-D 3
T also showed low DNA-DNA reassociation values (less than 21%) with respect to the three subspecies of
K. pneumoniae and
Klebsiella singaporensis. Its major fatty acids were C
14:0, C
16:0, c-C
17:0 and c-C
19:0 ω8c. The G+C content of the DNA was 58.3 mol%. Phylogenetic and phenotypic results supported the assignment of CW-D 3
T as a novel species of the genus
Klebsiella, for which the name
Klebsiella alba sp. nov. is proposed. The type strain is CW-D 3
T (=LMG 24441
T=KCTC 12878
T =CCTCC AB 206144
T).
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