The Gram-negative, aerobic, non-motile, non-spore forming, and rod-shaped bacterium designated as THG-T17
T was isolated from the soil of a ginseng field of Pocheon in Korea, and its taxonomic position was investigated by using a polyphasic approach. The growth of strain THG-T17
T occurred at 4−40°C and pH 4.0−9.0 with 1−2% (w/v) NaCl on nutrient agar. Strain THG-T17
T displayed β-glucosidase activity that was responsible for its ability to transform ginsenoside Rb
1 (one of the dominant ginsenosides of ginseng) to compound F2 via gypenoside XVII. On the basis of 16S rRNA gene sequence similarity, strain THG-T17
T was shown to belong to the genus
Pedobacter and was related to
Pedobacter soli 15−51
T (98.8%),
Pedobacter sandarakinus DS-27
T (98.0%) and
Pedobacter terrae DS-57
T (98.1%). The G+C content of the genomic DNA was 42.4 mol%. The DNA-DNA relatedness values between strain THG-T17
T and its phylogenetically closest neighbors were below 14%. Phenotypic and chemotaxonomic data, especially analysis of cellular fatty acid, supported the affiliation of strain THG-T17
T to the genus
Pedobacter. The results of genotyping and biochemical tests showed strain THG-T17
T to be differentiated genotypically and phenotypically from the recognized species of the genus
Pedobacter. Therefore, the novel isolate represents a novel species, for which the name
Pedobacter kyungheensis sp. nov. is proposed, with the type strain THG-T17
T (=KACC 16221
T = LMG 26577
T).
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