The Journal of General and Applied Microbiology
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Volume 61 , Issue 2
Showing 1-5 articles out of 5 articles from the selected issue
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  • Shilpa P. Shah, Kiran S. Kalia, Jagdish S. Patel
    Volume 61 (2015) Issue 2 Pages 35-43
    Released: May 27, 2015
    JOURNALS FREE ACCESS
    The purpose of this study was to produce a higher amount of cellulase by using an alternative carbon source, such as banana agrowaste, and to optimize the fermentation parameters for a high yield. In the present study, cellulase-producing Penicillium was isolated from a decaying wood sample. Different nutritional and environmental factors were investigated to assess their effect on cellulase production. The highest crude enzyme production was observed at a pH 6.0 and a temperature of 28°C in a medium that was supplemented with banana agrowaste as the carbon source. Pretreatment with 2N NaOH, at 7% substrate (banana agrowaste) concentration yielded the highest cellulase activity. Further to this, the effect of other parameters such as inoculum age, inoculum size, static and agitated conditions were also studied. It is concluded that Penicillium oxalicum is a powerful cellulase-producer strain under our tested experimental conditions using banana agrowaste as the carbon source.
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  • Akane Nojiri, Shinjiro Ogita, Yasuhiro Isogai, Hiromi Nishida
    Volume 61 (2015) Issue 2 Pages 44-49
    Released: May 27, 2015
    JOURNALS FREE ACCESS
  • Musu Zha, Jie Yu, Yong Zhang, Hongmei Wang, Na Bai, Yanting Qin, De Li ...
    Volume 61 (2015) Issue 2 Pages 50-56
    Released: May 27, 2015
    JOURNALS FREE ACCESS
    In this study, the lactic acid bacterial population of Qula cheese from the Gansu and Sichuan provinces of China were isolated and identified. Eight strains of Streptococcus thermophilus were isolated, of which five strains were selected for further characterization based on their fermentation properties. The changes in a number of parameters, including titration acidity, pH, viable counts, PrtS protease activity and the production of acetaldehyde, diacetyl and organic acid, were monitored during fermentation and the storage of fermented milks produced by the respective strain. All of the strains displaying acidifying capacity and all five fermented milks maintained high viable counts of S. thermophilus from fermentation to storage. Our study found that the changes in the monitored parameters were strain-specific and varied considerably among the five tested strains. Fermented milks produced by strain IMAU80809 had the highest concentration of acetaldehyde and were most favorable in the sensory evaluation. This study confirms that Qula cheese is a good source for isolating novel lactic acid bacterial strains with different fermentation properties, which will be very useful for further development and industrialization of traditionally fermented dairy products.
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  • Satoru Kaifuchi, Mihoko Mori, Kenichi Nonaka, Rokuro Masuma, Satoshi & ...
    Volume 61 (2015) Issue 2 Pages 57-62
    Released: May 27, 2015
    JOURNALS FREE ACCESS
    Screening for NADH-fumarate reductase inhibitors led to the isolation of a new ukulactone analog, ukulactone C, as a major polyene compound produced by Talaromyces sp. FKI-6713. The structure of the compound was elucidated as a reduced analog of ukulactone A by 1D- and 2D-NMR experiments. Ukulactone C possessed a potent inhibitory activity (IC50 = 62 nM) against NADH-fumarate reductase of the roundworm Ascaris suum in vitro.
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