Changes in the concentration of protein and free amino acids were examined in plasma, liver and muscle of rats fed high protein diets and the concentration ratios of free amino acids in liver and muscle to those in plasma were estimated to study the process of adaptation to high protein diets. Three groups of rats pretreated with 10 % egg albumin diet for 10 days were thereafter fed 10 % egg albumin diet (A
10), 50 % egg albumin diet (A
10→A
50) and 50 % soy protein diet (A
10→S
50) for 14 days. Other three groups of rats pretreated with 10 % soy protein diet for 10 days were fed 10 % soy protein diet (S
10), 50 % soy protein diet (S
10→S
50) and 50 % egg albumin diet (S
10→A
50) for 14 days.
Food intake was depressed initially in rats fed 50 % protein diets, especially in S
10→A
50 group. Free amino acid concentration in plasma, liver and muscle of the groups of 50 % protein diets varied during the first 7 days, but on the 14th day concentrations of most of them were almost the same as those values of each 10 % protein diet groups. In A10→A50 and A
10→S
50 groups the concentration ratios of free amino acids in liver to those in plasma. increased considerably on the 2nd day, but in S
10→A
50 and S
10→A
50 groups they varied slightly. Total protein content of liver increased from the 1st day in S10→S50 group, from the 7th day in A10→A50 and A10→S50 groups, while in S
10→A
50 group it decreased significantly up to the 7th day with body weight loss.
In these 4 groups of diets converting from low to high protein content S
10→A
50 group revealed remarkable decrease of food intake and seemed to retard an adaptation to high protein diet.
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