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Volume 12, Issue 4
Displaying 1-12 of 12 articles from this issue
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Yoshiko Shiraishi1966 Volume 12 Issue 4 Pages 183-187
Published: August 30, 1966
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSMethods for the separation and identification of carcinogenic polynuclear hydrocarbons in the smoke flavoring, 3, 4-benzpyrene (I), 1, 2, 5, 6-dibenzanthracene (II), and 3-methylcholanthrene (III) were investigated. n-Hexane extracts of the smoke flavoring were applied on an alumina column (0.9×15 cm, contained 1 per cent water), and the column was eluted with n-hexane-ether (1 : 1). Uuknown yellow substances in the n-hexane extracts were held on the top of the column, while the hydrocarbons were eluted out. This separation of hydrocarbons from interfering substances facilitated their subsequent identification. Thin layer chromatography was adopted for identification of (I), (II) and (III), using silica gel as adsorbent and n-hexane-o-dichlorobenzene-pyridine (10 : 1 : 0.5) as developer. The each spot was detected by the fluorescence under an ultra violet light (365mμ). The method was utilized for analysis of the commercial preparations.View full abstractDownload PDF (619K) -
Hiroshi Kozuka, Takeshi Niwase, Hideo Isono1966 Volume 12 Issue 4 Pages 187-190
Published: August 30, 1966
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSNeutron activation analysis of hair samples from 10 Japanese persons (5 males and 5 females) was performed to investigate the feasibility of establishing individuality. Hair samples were irradiated for 5 hours in a thermal neutron flux of 2×1012 n/cm2. sec. The gross gamma activity and the decay of the activity were determined, and gamma spectrometry was performed with a 256 channel pulse height analyzer without chemical separation. Micro contents of trace elements as Na, Mn, Cu, Br and Au were determined by the spectra. The patterns of spectra were slightly different each other in micro parts and useful for establishing individuality. The individuality was remarkable at the 0.41 MeV energy peak of gold. A simple way to individualize samples was to compare the patterns of spectra of samples which had resembling Na contents, as the Na content was correctly determined by the nondestructive method.View full abstractDownload PDF (505K) -
Yasuhiro Yamane, Koko Motomiya, Yoko Komiyama1966 Volume 12 Issue 4 Pages 191-195
Published: August 30, 1966
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSPrevious study has shown that red-fluorescent product was formed by reaction of vitamin B1 with cupric ion, and could be applied to the detection of vitamin B1. The present report deals with the synthesis and the structure of the product of vitamin B1 with copper, cadmium and zinc, respectively. In order to find the condition for the synthesis of the product, the effect of metal ion on the stability of vitamin B1 was examined by determining vitamin B1. When respective ion of copper (II), cadmium (II) and zinc (II) was reacted with thiolized vitamin B1 at pH 10∼11, vitamin B1 was decomposed only by copper vigorously at 37°and slowly at 0°, but the decomposition of vitamin B1 was hardly observed by any other metal under the experimental conditions. Therefore, their metal salts of vitamin B1 were synthesized as follows : vitamin B1-HCl solution (1 mol) was added into NaOH solution (3 mol) at room temperature, after about 45 minutes, the metal ion solution (1 mol) was added to the mixture at pH 10∼11 under cooling and stirring. The precipitate was recrystallized from iso-butanol or iso-propanol. The molar ratio of metal to vitamin B1 of the crystal was found to be 1 to 2 by chemical analysis. The measurements of U.V. and I.R. spectra of their salts showed the structure of metal chelate.View full abstractDownload PDF (617K) -
Kenji Sato, Nobuo Ito, Mitsuru Oka, Gisuke Meshitsuka1966 Volume 12 Issue 4 Pages 195-198
Published: August 30, 1966
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSThe application of radiation to the sterilization of drugs was studied using water soluble chlorophyll derivatives. Copper chlorophyll, methionine cobalt chlorophyll, and 5, 6-dimethyl benzimidazole cobalt chlorophyll, in solid state were irradiated with 60Co γ-rays. No detrimental effect on the chemical properties was observed at a dose of 3 Mrad. The sterility test was performed according to the direction in the Japanese Pharmacopoeia, Ed. VII. An adequate dose for the sterilization of chlorophyll derivatives is estimated to be 1∼2 Mrad.View full abstractDownload PDF (490K) -
Kenji Sato, Nobuo Ito, Mitsuru Oka, Gisuke Meshitsuka, Akio Shimohira, ...1966 Volume 12 Issue 4 Pages 199-202
Published: August 30, 1966
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSEffects of 60Co γ-rays irradiation on the median lethal dose, leukocyte mobilizing action and other pharmacological properties of chlorophyll derivatives were studied using male mice (DDY strain) and rabbits. Cu-chlorophyll, M-Co-chloropyll and DBI-Co-chlorophyll in solid state were irradiated with 3 Mrad 60Co γ-rays. The median lethal dose of the irradiated and nonirradiated Cu-chlorophyll by the intraperitoneal injection was determined to be 0.773±0.016 mg/g (body weight of mouse) and 0.769±0.016 mg/g, respectively. Intravenous administration of chlorophyll derivatives to normal rabbits caused a remarkable leukocytosis (mainly due to neutrophilia) at 3 to 5 hours after injection, and no significant difference in the leukocyte mobilizing action between the irradiated and nonirradiated samples was observed. From these results, the possibility of practical application of radiation to the sterilization of drugs was discussed.View full abstractDownload PDF (546K) -
Keiiti Sakai1966 Volume 12 Issue 4 Pages 202-204
Published: August 30, 1966
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSThe content of vitamin in K1 in rat liver was determined at various time intervals after the intraperitoneal injection of Vitamin K1. Seperation and purification of Vitamin K1 from liver extracts were carried out by alumina column chromatography follwed by thin layer chromatography. The spot on the thin layer plate was extracted and determined fluorometrically as reported previously. The amount of vitamin K1 in liver reached maximum within 3-6 hours after injection and became undetectable in 12-18 hours later. Any characteristic accumulation of this fat soluble vitamin was not observed in liver.View full abstractDownload PDF (473K) -
Yasuo Nakazawa, Toshiaki Osawa, Yutaka Uda, Noriko Matsumoto, Noriko I ...1966 Volume 12 Issue 4 Pages 204-207
Published: August 30, 1966
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSSeveral kinds of alkyl-and phenyl-mercury compounds were identified by thin-layer chromatography. Alumina layer was adopted to separate alkyl mercury homologue and EDTA-containing alumina plate was used for detection of ethyl-and phenyl-mercury compounds by developing with chloroform-methanol-water (80 : 20 : 2) in both cases. Ionophoretic conditions were further investigated to separatively identify these mercurial compounds.View full abstractDownload PDF (563K) -
Ryoji Sawamura, Tadashi Motoyama1966 Volume 12 Issue 4 Pages 207-213
Published: August 30, 1966
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSTurbidity, value of COD, contents of NH3-N, NO2--N, NO3--N and Cl- were measured on the water of several swimming pools with recirculation system, and the significance and variation of the results of each test and their mutual relationship were discussed. Any significant mutual relationship was not observed among them. The values of turbidity, COD and NO2--N were very changeable, though the value of COD were always under 3 ppm (12 ppm as for KMnO4 consumption value) owing to the control of recirculation system. The relation between turbidity and value of COD showed that there are two different KMnO4 consuming factors in the pool water, one of them is suspended material to be removed by recirculation and another one is soluble and not removed by recirculation. The results of successive tests showed that NH3-N, NO2--N and Cl- had a tendency to accumulate, except one swimming pool where NH3-N and NO2--N were not detected at all times.View full abstractDownload PDF (767K) -
Senji Suenaga1966 Volume 12 Issue 4 Pages 214-220
Published: August 30, 1966
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSKaolin has been used conventionally for preparing the standard solution of turbidity in the water analysis in Japan. But the variation of turbidity of kaolin suspension are remarkable owing to the origin of kaolin. Satisfactory standard was able to be prepared, when the particle size of kaolin was unified by sedimentation method. The standard solution prepared by this new method was much more stable than the conventional standard solution. The outline of the preparation is as follows : An adequate amount of kaolin is dispersed with 0.002 M sodium pyrophosphate solution, mixed thoroughly with homogenizer (8000 rpm), and settled in a tall cylinder for 24 hours. The upper turbid layer thus obtained is transferred to a tall cylinder and settled for about 100 hours. The upper layer is discarded and the residual precipitate is dispersed in a fixed volume of water. The standardization method of this suspension will be reported in the other papers.View full abstractDownload PDF (811K) -
Ai Watabe, Masako Tezuka, Taro Kawamura, Tetsuo Inoue, Kazuo Morishita ...1966 Volume 12 Issue 4 Pages 220-223
Published: August 30, 1966
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSAt present, oil red, yellow AB, OB, etc. are used as the staining reagents for detection of remaining fat on the tablewares. But recetly, some plastic tablewares have been used in mass feeding, and some of them are stained by these reagents. Therefore, some other reagent, by which only fat can stained, has been desired. It was found that, by using 0.1% ethanol solution of curcumin, only fat was stained without dyeing tablewares, and that fat could be detected very sensetively by yellowish green fluorescence under UV light. This reagent was applied for detection of fat and the results were compared with that of oil red. From the results of experiments, curcumin was found to be a more favorable reagent for detection of remaining fat.View full abstractDownload PDF (520K) -
Seitaro Iki, Hiroko Jozuka, Seiichi Sato1966 Volume 12 Issue 4 Pages 224-227
Published: August 30, 1966
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSIn this report, we experimented on the inner temperature of roast egg and foods which were heated by radarange. We found both of them have a character that temperature of central portion becomes higher than the surface, and central temperature is not uniform.View full abstractDownload PDF (545K) -
Tokuichi Miyanaga1966 Volume 12 Issue 4 Pages 227-230
Published: August 30, 1966
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSEffects of pH values on the amounts of chloride determined by five analytical methods were investigated. In titration analysis, a particular error was respectively shown by Mohr, Volhard, and mercuric nitrate method. Uranine solution was unsuitable as the indicator, since remarkable end point was not given. In colorimetric determination by mercuric thiocyanate, however, no difference of color tone was visually observed by various pH values of the solution containing 50 ppm chlor ion.View full abstractDownload PDF (557K)
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