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Volume 20 (1974)
- Issue 6 Pages 305-
- Issue 5 Pages 239-
- Issue 4 Pages 179-
- Issue 3 Pages 113-
- Issue 2 Pages 47-
- Issue 1 Pages 1-
Volume 20, Issue 4
Displaying 1-11 of 11 articles from this issue
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[in Japanese], [in Japanese], [in Japanese]1974 Volume 20 Issue 4 Pages 179-193
Published: August 31, 1974
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSDownload PDF (1393K) -
NORIHIDE NOSE, AKIO TANAKA, SUSUMU KOBAYASHI, TOSHIMASA SUZUKI, YOJI H ...1974 Volume 20 Issue 4 Pages 194-199
Published: August 31, 1974
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSGas chromatographic determination of 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (furyl furamide) in food was established as follows. A mixture of more than 1 μg of furyl furamide, 10 μl of hepta fluorobutyric anhydride (HFB), and 0.5 ml of acetone in a 25 ml light-resistant glass test tube is allowed to stand for 5 min at room temperature. After removal of unreacted HFB with a capillary tube under a reduced pressure, acetone is added exactly to the volume indicated. The sample so obtained is subjected to gas chromatography. The lower limit of the sample size of furyl furamide for identification was 1 ng. The method presented here may be applied to determine furyl furamide contained in food as a fungicide.View full abstractDownload PDF (594K) -
MASAYOSHI YAMAGUCHI, KAZUYO SAIDO, TAKEO YAMAMOTO1974 Volume 20 Issue 4 Pages 200-203
Published: August 31, 1974
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSIn order to elucidate the mechanism of liver disorders in lead poisoning, alkaline phosphatase activity in rat liver was examined after a single intraperitoneal administration of lead. The serum alkaline phosphatase activity showed the lowest level on 5 th day after injection of lead, increased, thereafter, and returned to the normal level 10 days. On the other hand, liver alkaline phosphatase value was lower on 4 th day being 75% of that of normal rats, and this lowered level continued until 30 th day after treatment with lead. The decrease of hepatic alkaline phosphatase activity was in proportion to the amount of lead administered. In the administration of tin, zinc, manganase, calcium, or lead, the decrease of hepatic alkaline phosphatase activity was the largest in the case of treatment with lead, and the activity did not change by the administration of calcium or zinc. The amount of calcium in the liver increased rapidly after the administration of lead, and calcium at 30 days was approximately 8 times compared with the normal level.View full abstractDownload PDF (587K) -
NORIKAZU KOJA, KENSHU MOCHIDA1974 Volume 20 Issue 4 Pages 204-208
Published: August 31, 1974
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSRicin, which has a highly lethal toxicity and pyrogenic activity, was obtained in a homogeneous state from seeds of Ricinus communis by the method of Ishiguro, et al., with a little modification. Some physicochemical properties of the purified ricin were examined and the values obtained were as follows : Sedimentation constant, S20, 4.70×10-13 ; isoelectric point obtained by the electrofocusing fractionation, pH 7.30, estimated molecular weight, 53000-54000. Treatment of ricin with 2-mercaptoethanol dissociated it into two subunits with molecular weight of ca. 24000 and 30000. Chemical analysis suggested its sugar content to be 6.7% as hexose, and ricin seemed to be made up of 18 kinds of amino acid, there being no evidence for the presence of an abnormal amino acid (s). Pyrogenic activity of ricin was characterized by a long latent period and duration of fever for a long period of time, the amount of fever increased in proportion to the dose, and dose-activity correlation existed in the range of 0.05-0.2 μg/ml/kg.View full abstractDownload PDF (755K) -
NORIKAZU KOJA, KENSHU MOCHIDA, MINORU YOSHIDA1974 Volume 20 Issue 4 Pages 209-212
Published: August 31, 1974
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSThe pyrogenic property of purified ricin obtained from the seed of Ricinus communis was studied in comparison with bacterial pyrogen (LPS) obtained from E. Coli UKT-B. Ricin produced a durable fever in rabbits by intravenous or subcutaneous injection and caused leucopenia, but the secretion of endogenous pyrogen was not found in their serum. Response of pyrogenicity of ricin was decreased in leucopenic rabbits induced by nitrogen mustard. Tolerance to the pyrogenic effect of ricin was enhanced by its repeated subcutaneous injection but there was no evidence for cross tolerance between ricin and LPS. These results suggested that the mechanisms by which purified ricin exerts the pyrogenic effect may be different from that of LPS.View full abstractDownload PDF (554K) -
MASATOSHI SYOYAMA, YUZO MIYACHI1974 Volume 20 Issue 4 Pages 213-216
Published: August 31, 1974
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSMetabolism of 3, 5-dimethylphenyl N-methylcarbamate (Cosban) was examined. Cosban was administered to rabbits during 1 week and urine was collected and extracted daily. From the ether extract under acidic condition, 5-hydroxytoluic acid and (3-carboxy-5-methyl) phenyl N-methylcarbamate were isolated and identified with authentic samples by mixed melting point determination, and infrared and nuclear magnetic resonance spectra. 3, 5-Dimethylphenol was detected by thin-layer chromatography (TLC) and gas chromatography. The aqueous layer left after ether extraction was extracted with a mixed solvent of ether and ethanol (2 : 1) and, from this extract, glucuronides of both 5-hydroxytoluic acid and 3, 5-dimethylphenol, and sulfate of 3, 5-dimethylphenol were obtained. To 100 ml of human urine, 1 mg each of Cosban, (3-carboxy-5-methyl) phenyl N-methylcarbamate, 5-hydroxytoluic acid, and 3, 5-dimethylphenol were added and the mixture was kept overnight. These substances were well separated and detected by silica gel TLC using a developing solvent of hexane-ethyl acetate-formic acid (75 : 25 : 5) and a coloration reagent of 2, 6-dibromoquinone chloroimide.View full abstractDownload PDF (556K) -
TETSUO INOUE, MASAYOSHI TATSUZAWA, SHIGEKO HASHIBA1974 Volume 20 Issue 4 Pages 217-222
Published: August 31, 1974
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSColorimetric determination of pheneacetin and Bucetin in mixed pharmaceutical preparations was examined. Phenacetin and Bucetin react with nitric acid-acetic acid -0.1% sodium nitrite (40 : 10 : 0.1) solution to give nitrophenacetin and nitrobucetin, respectivly. Nitrophenacetin and nitrobucetin were each hydrolyzed with sodium hydroxide to produce nitrophenetidine which was extracted with chloroform and showed absorption maximum at 435 nm. Presence of acetaminophen, salicylamide, and potassium guaiacolsulfonate does not interfere with this method.View full abstractDownload PDF (637K) -
TETSUO INOUE, MASAYOSHI TATSUZAWA, MASAMI HOJO1974 Volume 20 Issue 4 Pages 223-227
Published: August 31, 1974
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSColorimetric determination of aminopyrine in mixed pharmaceutical preparations was examined. Aminopyrine reacts with ferric chloride and dipyridyl to give a red color, with absorption maximum at 525 nm. Presence of sulpyrine and salicylate interfered with this determination but they could be eliminated by extraction with carbon tetrachloride.View full abstractDownload PDF (509K) -
TETSUO INOUE, MASAYOSHI TATSUZAWA, HIDEKO TAKABE1974 Volume 20 Issue 4 Pages 228-232
Published: August 31, 1974
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSColorimetric determination of bromvalerylurea in mixed pharmaceutical preparations was examined. Bromvalerylurea reacts with acetylacetone and p-dimethylaminobenzaldehyde at 70° to give a green color having an absorption maximum at 595 nm. Presence of caffeine, sulpyrine, and thiamine interfered with this determination, but they could be eliminated by fractional extraction.View full abstractDownload PDF (505K) -
MASAKAZU DEGUCHI, YOSHITAKE SUMIDA, MIKIO YASHIKI1974 Volume 20 Issue 4 Pages 233-235
Published: August 31, 1974
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSPyrocatechol violet was applied as a reagent for the spectrophotometric determination of tin in canned foods. The sample of canned food was first decomposed with a mixture of sulfuric and nitric acids, and tin in resulting solution was determined by a method reported by Tanaka, et al. using Pyrocatechol violet. Analytical results of several canned foods were in good agreement with those obtained by SATP method. Good results were obtained for the recovery test of tin in canned foods.View full abstractDownload PDF (387K) -
MASAYOSHI YAMAGUCHI, MASAHIKO NISHIZAWA, TAKEO YAMAMOTO1974 Volume 20 Issue 4 Pages 236-238
Published: August 31, 1974
Released on J-STAGE: May 30, 2008
JOURNAL FREE ACCESSThe amount of calcium in the liver of rats was examined after continuous oral administration of 0.1 mg/day of lead or cadmium acetate for 30 days, and the following results were obtained. The amount of calcium in the liver increased about 3 times (p<0.01) in the lead-treated group, but decreased to about one-half (p<0.01) in the cadmium-treated group compared with control group. On the other hand, the liver calcium concentration in both lead and cadmium-treated group increased significantly compared with that of the cadmium-treated group. These data suggest that lead antagonizes the effect of cadmium on the amount of calcium in the liver of rats.View full abstractDownload PDF (481K)
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