Journal of Insect Biotechnology and Sericology
Online ISSN : 1884-7978
Print ISSN : 1346-8073
ISSN-L : 1346-8073
80 巻, 2 号
選択された号の論文の5件中1~5を表示しています
Regular Articles
  • Masahiko Hirokawa, Ken-Ichiro Tatematsu
    2011 年 80 巻 2 号 p. 2_041-2_048
    発行日: 2011年
    公開日: 2012/06/01
    ジャーナル フリー
    The Nc-mutant gene and E pseudoalleles of Bombyx mori correspond to the Antennapedia and bithorax complexes of Drosophila melanogaster, respectively. The arrangement of the Nc-mutant gene and three mutant genes, ECw, EKp and EMu, belonging to the E-pseudoallelic genes located on the sixth-linkage group, was investigated by genetic analysis. It indicated that the arrangement of the four mutant genes is in the order of EMu-EKp-ECw-Nc from the proximal side of the sixth-linkage group, and genetic distances between adjacent loci were calculated to be 0.72 centimorgans (cM) between EMu and EKp, 0.57cM between EKp and ECw, and 2.20cM between ECw and Nc. The order of these genes on the chromosome corresponds to the order of segments in which each mutant gene primarily expresses the mutant characteristics along the anteroposterior axis, in a similar way to those of Drosophila melanogaster and Tribolium astaneum.
  • Asahi Suzuki, Yasutaka Yoshizawa, Shiho Tanaka, Madoka Kitami, Ryoichi ...
    2011 年 80 巻 2 号 p. 2_049-2_056
    発行日: 2011年
    公開日: 2012/06/01
    ジャーナル フリー
    It has been suggested that early defense mechanisms of the silkworm Bombyx mori, which can clear 106 bacterial cells from the hemolymph within 30minutes, largely depend on pathogen recognition mediated by dual-carbohydrate recognition domain (CRD) C-type lectins and nodule formation by hemocytes. In order to understand this phenomenon, the present study explores both inter- and intracellular signal transduction pathways in association with nodule formation by hemocytes of B. mori. The C-type lectin inhibitors dithiothreitol and ethylenediaminetetraacetic acid inhibited in vivo nodule formation, and a dual-CRD C-type lectin was required for in vitro nodule-like aggregation. These results suggested that dual-CRD C-type lectins were required as pathogen-recognition receptors for nodule formation. Serine protease inhibitors such as benzamidine hydrochloride, aprotinin, and leupeptin inhibited nodule formation, and thrombin, a serine protease, induced in vivo nodule-like aggregation. This indicates that serine proteases behave as mediators in the extracellular signal cascade leading to nodule formation. An inhibitor of phospholipase C, U-73122, inhibited both in vivo nodule formation and in vitro nodule-like aggregation. EDTA and pertussis toxin inhibited in vivo nodule formation. These results suggest that the cell reaction triggered by the two G protein-mediated signal transduction pathways plays a significant role in nodule formation.
  • Kazuhiro Iiyama, Jae Man Lee, Takahiro Kusakabe, Chisa Yasunaga-Aoki, ...
    2011 年 80 巻 2 号 p. 2_057-2_061
    発行日: 2011年
    公開日: 2012/06/01
    ジャーナル フリー
    We constructed pHERDHis vectors to express polyhistidine-tagged proteins based on the pHERD vectors. The oligonucleotides containing the hexahistidine sequence were designed to be able to introduce the tag at either the NH2- or COOH- terminal. To demonstrate the usefulness of the vectors, a pyocyanin biosynthetic protein, PhzS, was expressed in a pyocyanin-deficient mutant of Pseudomonas aeruginosa. In the mutant strain, both HisPhzS (tag at the NH2- terminal) and PhzSHis (tag at the COOH- terminal) worked as a functional protein and pyocyanin was produced. It was also demonstrated that HisPhzS was purified by standard affinity chromatography.
  • Sukunya Chumchuen, Rensuke Kanekatsu
    2011 年 80 巻 2 号 p. 2_063-2_070
    発行日: 2011年
    公開日: 2012/06/01
    ジャーナル フリー
    Amplified fragment length polymorphism (AFLP) analysis was used to measure the genetic diversity of the genus Morus among cultivated mulberry plants of different origins and naturally occurring wild types. A total of 105 clearly visible amplified bands were separated on a polyacrylamide gel, and 27 bands (24.58%) of them were found to be polymorphic. The genetic similarity coefficient based on the AFLP ranged from 0.556 to 0.992 between the mulberry cultivars themselves, and from 0.603 to 0.865 between the mulberry cultivars and the wild type mulberries. The cophenetic correlation coefficient was r=0.9606, indicating a high statistical agreement between the dendrogram clusters and the similarity matrix. Cluster analysis of the AFLPs using the unweighted pair group method with an arithmetic average (UPGMA) revealed a genetic distance between the mulberry cultivars and wild type accessions, supporting the UPGMA clustering. The results of this study showed that AFLP analysis is a useful tool for investigating the genetic diversity of the genus Morus.
  • Keisuke Mase, Chikara Hirayama, Eiji Okada, Tetsuya Iizuka, Keiko Kado ...
    2011 年 80 巻 2 号 p. 2_071-2_077
    発行日: 2011年
    公開日: 2012/06/01
    ジャーナル フリー
    The inheritance pattern of the green cocoon of Bombyx mori has been complicated because the cocoon color trait seems to be in continuous variation. In the present study, the degree of green in the cocoon shell was quantitatively evaluated in the BC1 and BC2 segregations between the green and white cocoon strain by chromaticity. Furthermore, linkage analyses of the faint-green cocoon were performed on the BC1 and BC2 segregants using PCR-amplified length polymorphisms based on the SNP map, which is unified with a classical linkage map. Consequently, a new marker gene associated with the faint-green cocoon appearance was identified on the 27th linkage group in which a former unique morphological marker (Xan) was lost because of the uniformity of a marker (Sel) on the 24th linkage group.
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