Journal of Insect Biotechnology and Sericology
Online ISSN : 1884-7978
Print ISSN : 1346-8073
ISSN-L : 1346-8073
82 巻, 2 号
選択された号の論文の4件中1~4を表示しています
Regular Articles
Short Communication
  • Koji Kitaguchi, Rina Hamajima, Hayato Yamada, Michihiro Kobayashi, Mot ...
    2013 年 82 巻 2 号 p. 2_049-2_054
    発行日: 2013年
    公開日: 2013/12/05
    ジャーナル フリー
    Ld652Y cells from the gypsy moth, Lymantria dispar, are highly sensitive to apoptotic stimuli compared to BM-N cells from the silkworm, Bombyx mori, and undergo apoptosis upon infection with various nucleopolyhedroviruses (NPVs). We previously cloned and characterized Dronc homologues from L. dispar Ld652Y and B. mori BM-N cells and demonstrated that L. dispar Dronc (Ld-Dronc) undergoes proteolytic processing more rapidly and extensively than does B. mori Dronc (Bm-Dronc) upon infection with vAcΔp35, a p35-defective Autographa californica multiple NPV. Here, we comparatively examined expression and proteolytic processing of the Ld- and Bm-Dronc proteins in the transient expression assays using three different insect cell lines, Ld652Y, BM-N and Spodoptera frugiperda Sf9. We demonstrate that even in the transient expression assays, Ld-Dronc undergoes proteolytic processing and activates caspase-3-like protease more rapidly and extensively than Bm-Dronc in a cell-line independent manner. These results indicate that intrinsic properties of Dronc are involved in the differing capacity of Ld652Y and BM-N cells to induce apoptosis in response to apoptotic stimuli, including NPV infection.
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