The cell density of
Pavlova lutheri was measured during a 17 days-long culture period at the three different-sized culture vessels; a 3
l flask, a 20
l tank, and a 100
l tank. PPFD (Photosynthetical Photon Flux Density) at the center of the 3
l flask and the 20
l tank under fluoresent lamps was 45μmol· m
-2·s
-1, while that at the 100
l tank was 300μmol·m
-2·s
-1 under high pressure sodium lamps, and 0-1300μmol·m
-2·s
-1 under the natural sun light. The cell density was 2.1×107 cells·m
l-1 at the 3
l flask, 7.4×107 cells·m
l-1 at the 20
l tank, and 1.6×107 cells·m
l-1 at the 100
l tank. Differences at the cell density were found in between the 3
l flask and the 20
l tank, though the same light sources were used for these two systems. The cell densities at the 100
ltank were also different when high pressure sodium lamps and natural sun light were used, however no difference at the cell density was found between the 100
l tank under high pressure sodium lamps and the 3
l flask under fluorescent lamps. Through these results, it can be concluded that a propagation rate of
P. lutheri increases under higher PPFD. For higher scaling up of vessels will increase under higher PPFD lighting. It is necessary to examine the much more efficient lighting in order to maximize a propagation rate of the micro algae.
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