In this paper, the toxicity of PCP-Na for the “Dojo” fish was discussed through results obtained by a regression method, taking into consideration the immersion time of the fish and the concentrations of the testing solution. The fish ranged from 0.4 to 12.7g in weight. Their weights were determined by taking the difference in weight of a vial with testing solution and the same vial with solution and a fish. Data on dead or alive were recorded individually. Immersion time in the testing solution was controlled by washing the whole test vial, including the fish, in running water. From the data of 87 combinations of concentrations (ppm) per weight (g) of fish
C and time of immersion
T (min.), together with indications of whether or not the fish responded, an equation for fitting a probit regression plane was estimated as:
Y=-5.1725+3.3485x
1+5.6678x
2. Here x
1 and x
2 are log
C and log
T respectively and
Y is the probit probability of response
P. Results of the χ
2 test showed no significant difference between the empirical probits and the predictions from the fitted equation. The 5% fiducial limit curves (
t=1.96) for
P=0.5 and 0.9 were calculated and were shown in the figures.
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