To evaluate the effect of chilling on mass-reared melon fly, Bactrocera cucurbitae COQ., groups of adult flies were exposed to 3, 0.5, -2.2 and -3.5°C for 6, 12, 24 and 48h. The recovery and longevity of adult chilled for less than 24h at about 0.5°C was not adversely affected. A special container for chilled flies, which was able to keep the temperature below 10°C for 4h, was designed for their long-distance transport. The longevities of flies using aerial distribution by helicopter and hand release on the ground using the chilled transport container were compared with direct release from an emergence box without chilling at Miyagi Island in Okinawa Prefecture. There were no significant differences in longevity between the three release methods.
A simplified small apparatus for extracting nematodes was designed and its extraction efficiency was tested using Bursaphelenchus xylophilus in dead Pinus densiflora wood. The extraction efficiency of the simplified apparatus was not significantly different from that of an ordinary 9-cm diameter BAERMANN funnel when the sample amount was appropriate. The simplified apparatus is small (ca. 3cm×3cm×10cm) taking up about 5% of the space required by the 9-cm BAERMANN funnel. It is expected to be useful when extracting nematodes in a small chamber or when treating large numbers of samples at one time.
The mating behavior of the eggplant fruit-borer was observed in the laboratory under natural light conditions. Copulation occurred around 04:40 and the duration was 73 minutes. Virgin-female traps captured males both in a large-field cage, and in an eggplant field. Males were trapped between 04:00 and 05:00 in the field. This result coincided with the time of copulation observed in the laboratory.