The effectiveness of biological control of
Liriomyza trifolii (Burgess) by the release of
Diglyphus isaea (Walker) on tomatoes was evaluated in greenhouses. Three experiments were tried in spring (Trial 1), early summer (Trial 2), and mid-summer (Trial 3). The parasitoid release rates were 0.13 _??_ per plant (5 releases) in Trial 1, 0.19 _??_ per plant (8 releases) in Trial 2, and 0.15 _??_ per plant (3 releases) in Trial 3. The host release rates were 0.5 _??_ per plant (1 release) in Trial 1, 0.5 _??_ per plant (3 releases) in Trial 2, and 0.5 _??_ per plant (1 release) in Trial 3. The densities of leafminer larvae in greenhouses where parasitoids were released in Trials 1, 2 and 3, were held below 1/4, 1/36, and 1/10 of the control larval density, respectively. The mortalities of leafminer larvae observed in Trials 1, 2 and 3, increased by up to 94.1%, 98.4%, and 100%, respectively. The number of empty mines in Trials 1, 2 and 3, were 1.3 per leaf, 2.2 per leaf, and 3.4 per leaf at the end of the experiment, respectively. The number of fallen leafminer pupae in trays under tomato plants in Trials 1 and 2, and the number of leafminer adults caught by yellow sticky traps in Trial 3, were few compared to the controls. The only parasitoid species emerging from
L. trifolii larvae in Trials 1, and 3 was
D. isaea, while the indigenous parasitoid,
Neochrysocharis formosa (Westwood), was dominant in Trial 2. The maximum percentage parasitisms based on the number of adult parasitoids emerging from samples collected in the release greenhouses in Trials 1, 2, and 3, were 92.3%, 86.7%, and 95.1%, respectively. Consequently, it is suggested that
D. isaea is effective against
L. trifolii on tomato in greenhouses from spring through summer.
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