We investigated to determine how β3AR (W64R), UCP1 (A-3826G), AGT (M235T) and MTHFR (C677T) polymorphisms effect on blood pressure, body mass index (BMI), glycemic control and serum lipids levels in Japanese patients with type 2 diabetes mellitus( T2DM). A total of 157 patients were screened for the variants of SNPs. The β3AR, UCP1, AGT and MTHFR, polymorphisms were analyzed by PCR-RFLP, and BMI, blood pressure, HbA1c, and serum lipids were examined. The patients who carry either or both β3AR (WR/RR) and UCP1 (AG/GG) genotypes demonstrated the higher BMI than patients with β3AR (WW) and UCP1 (AA) genotypes. Male patients with β3AR (WR/RR) and/or UCP1 (AG/GG) genotypes had more frequently had high systolic blood pressure than those with β3AR (WW : wild) and UCP1 (AA : wild) genotypes. Age and the existence of β3AR (WW/ RR) and/or UCP1 (AG/GG) genotypes were independent factors significantly associated with systolic blood pressure by multivariate analysis in male patients with T2DM. Blood pressure did not show AGT genotype-specific difference. However, female patients with AGT (TT) genotype showed higher mean HbA1c levels than females with AGT (MM/MT) genotypes. The AGT (TT) genotype was an independent factor significantly associated with HbA1c levels in female patients by multivariate analysis. Thus, gender is a very important factor in the effects of polymorphism on HbA1c, BMI, and blood pressure in Japanese patients with T2DM.

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