日本食品化学学会誌
Online ISSN : 2189-6445
Print ISSN : 1341-2094
ISSN-L : 1341-2094
12 巻, 2 号
選択された号の論文の9件中1~9を表示しています
論文
  • 玉野 静光, 河部 真弓, 畑原 百合子, 佐野 真土, 萩原 昭裕, 福本 隆行, 中村 幹雄, 白井 智之
    原稿種別: 本文
    2005 年 12 巻 2 号 p. 63-70
    発行日: 2005/08/31
    公開日: 2017/12/01
    ジャーナル フリー
    F344/DuCrj(Fischer)系ラットを用いて、ガラナ抽出物の13週間反復投与毒性試験を飼料中濃度0, 0.625, 1.25, 2.5および5.0%で実施した。臨床症状、体重、摂餌量、食餌効率、尿検査、血液検査、血液生化学的検査、眼科学的検査および肉眼的病理検査ではガラナ抽出物投与の影響を認めなかった。ガラナ抽出物投与による毒性学的意義のある変化は以下の通りであった。1.摂水量では、雌の2.5%以上の投与群において投与期間を通じて高値を示した。2.唾液腺では相対重量が雌雄の5.0%群で増加を認めた。3.病理組織学的検査では、顎下腺および舌下腺腺房細胞の肥大の発生を雌雄の2.5%以上の投与群で認めた。以上の結果より、ガラナ抽出物の13週間反復経□投与により、上記変化を認めなかった1.25%投与群(雄で940mg/kg/day、雌で949ms/ke/day)(caffeine 35mg/kg/dayに相当)を無毒性量(NOAEL)と判断した。
  • 田中 理恵, 森本 隆司, 中村 幹雄, 合田 幸広
    原稿種別: 本文
    2005 年 12 巻 2 号 p. 71-75
    発行日: 2005/08/31
    公開日: 2017/12/01
    ジャーナル フリー
    A food additive, "Methyl Hesperidin" is a mixture of methylation products of hesperidin. In order to clarify the components of the commercial food additives, we isolated four main compounds and identified them as 3'-monomethylhesperidin, 3',5-dimethylhesperidin, 2', 3, 6'-trimethylhesperidin chalcone and 2', 3, 6'-trimethyl-4'-(rhamnosyl-2-O-methylglucosyl)-hesperetin chalcone, by spectroscopic analyses. It is revealed that the molecular extinction coefficients of these compounds are almost the same at UV297nm. Therefore, we propose the quantitative method by utilizing UV absorption at 297nm for the specification of the food additive.
  • 丸山 卓郎, 酒井 信夫, 川原 信夫, 合田 幸広
    原稿種別: 本文
    2005 年 12 巻 2 号 p. 76-79
    発行日: 2005/08/31
    公開日: 2017/12/01
    ジャーナル フリー
    A commercial alkanet color was investigated using chemical and molecular biological methods. In our previous study, five major pigments were isolated from the color and identified to be shikonin/alkannin and their alkyl esters. In this study, the hydrolysates of these pigments were analyzed using chiral phase HPLC-CD. As a result, we confirmed that the principal pigments of the color are shikonin and its alkyl ester derivatives. The DNA sequences of the nuclear rDNA ITS1 region for the authentic plants of Anchusa officinalis and Borago officinalis and the source plant of the color were analyzed. The resulting sequence alignment clarified that the DNA sequences of the authentic plants are identical to the corresponding sequence in the database. On the contrary, the DNA sequence of the source plant is not identical with that of A. officinalis, although it is similar to those of the Boraginaceus plants, such as Echium vulgare, Ogastemma pusillum, and so on. Therefore, it seems that the original plant for the color is a Boraginaceus plant other than A. officinalis.
  • 伊藤 幸彦, 申 曼珍, 喜瀬 光男, 速水 耕介, 芳野 原, 吉原 理恵, 横山 淳一
    原稿種別: 本文
    2005 年 12 巻 2 号 p. 80-84
    発行日: 2005/08/31
    公開日: 2017/12/01
    ジャーナル フリー
    Effects of pre-germinated brown rice (PGBR) on postprandial blood glucose and insulin concentrations were compared with white rice (WR) in 16 (8 women and 8 men) subjects with hyperglycemia. We investigated the time course of postprandial blood glucose and insulin concentrations after ingesting PGBR or WR. The study was a randomized, cross-over design in which subjects participated in two separate 3-hour meal tolerance test. The tolerance tests were spaced more than 7 days apart for each subject. All the rice samples contained 50 g of available carbohydrates. The previous day the subjects ate the assigned dinner by 9:00 pm and then were allowed only water until the examination. The next morning, they ingested each test rice sample with 150ml of water in 5-10 min. Blood was sampled from the cubital fossa vein at 0, 30, 60, 90,120 and ISOmin after the ingestion. The postprandial blood glucose after ingesting PGBR was lower than WR, and the values of 60, 90,180 min were significantly lower (p<0.05). The incremental area under the curve (IAUC) of blood glucose concentrations for 180 min after the administration of PGBR was lower than that of WR (p<0.05). The postprandial blood insulin after ingesting PGBR was lower than WR, but not significant. IAUC of blood insulin concentrations of PGBR is tended lower compared with that of WR (p=0.07). These results suggest that intake of PGBR instead of WR is effective for the control of postprandial blood glucose concentration without increasing the insulin secretion in subjects with hyperglycemia.
ノート
  • 金 哲龍, 多田 敦子, 杉本 直樹, 佐藤 恭子, 山崎 壮, 棚元 憲一
    原稿種別: 本文
    2005 年 12 巻 2 号 p. 85-87
    発行日: 2005/08/31
    公開日: 2017/12/01
    ジャーナル フリー
    Fish scale foil is used as a natural food colorant. As the commercially available product is a suspended argent liquid, we analyzed the solvent and the particles, respectively. In result, the solvent was identified as ethanol by HS-GC/MS, and the suspended particles were confirmed by PDA-LC/MS to be mainly three purine derivatives, namely guanin, hypoxanthine, and xanthine. Their concentrations were 79.38%, 1.64% and 2.01% in the particles, respectively, by the absolute curves of the standards.
  • 豊嶋 尚美, 大張 靖幸, 高山 容子, 細川 晶, 木村 愼太郎, 伊藤 誉志男
    原稿種別: 本文
    2005 年 12 巻 2 号 p. 88-92
    発行日: 2005/08/31
    公開日: 2017/12/01
    ジャーナル フリー
    The conditions of the coupling reaction in the method of examining USAA, impurities of Food Coloring Matter indicated by JSFA, were studied on and improved as following. 1) The rate of coloring about the Food Coloring Matter rose by about 3 times, changing sodium carbonate solution concentration 10% to 30%. Moreover, the Food Coloring Matter Aluminum Lake rose by about 5 times, changing sodium carbonate solution concentration 10% to 60%. 2) The temperature had no influence on the rate of coloring during the reaction. 3) The reaction wasn't completed in 15 minutes, when using 10 ml of 10% sodium carbonate solution, on the condition of measure in JSFA. However, when 10 ml of 30% sodium carbonate solution was used, which is the solution concentration we improved, the reaction was completed in the 15 minutes. 4) The recovery test was examined on the conditions we improved, from the beginning of method process on the Food Coloring Matter. The recovery rate ascended in any color, Food Yellow No. 4, Food yellow No. 5, Food Red No. 2, Food Red No. 102, and Food Red No. 40. The reproducibility also became good. (See figure.) On the other hand, the recovery rate of Food Red No. 40 Aluminum Lake rose a little. Therefore further examinations, not only on coloring process but other processes are necessary. Abbreviations : USAA : Unsulfonated primary aromatic amines JSFA : Japan's Specifications and Standards for Food Additives seventh edition, including changes published on February 27, 2004. [figure] Fig.1 Comparison of recovery rates of JSFA method and Improved method (N=3, means with min. and max.)
  • 大野 弘美, 田村 幸一, 寺尾 由香, 阿武 尚彦
    原稿種別: 本文
    2005 年 12 巻 2 号 p. 93-99
    発行日: 2005/08/31
    公開日: 2017/12/01
    ジャーナル フリー
    The effect of Mallotus japonicus Muell. Arg. cortex water extract on defecation frequency and fecal characteristics was studied in 33 healthy women subjects with constipation tendency. The experimental duration (52 days) was divided into 4 phases : pre-ingestion period (2 weeks), un-observation period (10 days), ingestion period (2 weeks) and post-ingestion period (2 weeks). The subjects were divided into 3 groups after pre-ingestion period. Four capsules containing Mallotus japonicus water extract or not containing (placebo) were taken per day for 2 weeks. Two type of capsules for Mallotus japonicus were different content of extract, each intake of extract was 300mg (F300) or 550mg (F550) per day. Increase in defecation frequency and fecal amount was observed during ingestion period compared with pre-ingestion period in F300 and F550 groups, however in placebo group wasn't significantly. In F550 group defecation frequency was decreased significantly after discontinuation of the ingestion, however in placebo and F300 groups wasn't significantly. These results demonstrated that the ingestion of 300mg Mallotus japonicus water extract per day for 2 weeks improve defecation frequency and fecal amount of healthy women with a tendency of constipation. The relation of fecal amount and length of ingestion suggested that the ingestion of low dose act better as laxative by continuance.
  • 小嶋 美穂子, 福永 健治, 西山 利正, 原田 浩之, 瀧野 昭彦, 辻 元宏
    原稿種別: 本文
    2005 年 12 巻 2 号 p. 100-106
    発行日: 2005/08/31
    公開日: 2017/12/01
    ジャーナル フリー
    滋賀県における農産物中の残留農薬調査結果を1994年から2003年までの10年間についてまとめた。検体に対する検出率は、年により異なるが20%前後であった。野菜と果実を比較すると果実からの検出率が高い傾向にあり、国産と輸入を比較すると国産からの検出率が高かった。検出率の高い農薬は、国産農産物では、メタミドホス、アセフェート、シペルメトリン、フェンバレレート、輸入農産物ではクロルピリホス、メタミドホスであった。ピレスロイド系の農薬の検出率が高いが、10年間の経年変化をみると調査後半で減少傾向を示していた。農産物別に検出頻度を集計したところ、フェンバレレートはほとんどの農産物で上位であった。また、こまつな、しゅんぎく、ほうれんそう、ねぎは、シペルメトリンの頻度が非常に高かった。検出農薬数別に集計したところ、10年間で1農産物から1農薬のみの検出例は82%、2農薬は15%、3農薬は2%、4農薬は1%であった。検出された農薬で食品衛生法に残留基準が設定されている農薬は69.5%であった。それ以外の農薬は、残留基準が設定されていなかった。また、残留基準値を超過した事例は、10年間で3件であった。農薬の最大検出値を用いて農薬の摂取量を算出しADIと比較した。すべてADIの20%以下であった。
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