日本食品化学学会誌
Online ISSN : 2189-6445
Print ISSN : 1341-2094
ISSN-L : 1341-2094
24 巻 , 1 号
選択された号の論文の6件中1~6を表示しています
論文
  • 髙橋 美津子, 桜井 克巳, 菅谷 なえ子, 刈込 髙子, 斉藤 貢一
    2017 年 24 巻 1 号 p. 1-9
    発行日: 2017年
    公開日: 2017/04/29
    ジャーナル フリー
    To verify the presence of yohimbine and its four stereoisomers (corynanthine, α-yohimbine, β-yohimbine, and isorauhimbine) in health food and to perform a quantitative analysis, we examined the separation and detection conditions for each component by GC-MS and LC-UV-MS. As a result, in GC-MS analysis, baseline separation of all five components (yohimbine and its four stereoisomers) was achieved with a mid-polar column. However, the presence of a water desorption peak due to heating, calibration curves with poor linearity, and low recovery rates suggested that the use of GC-MS for quantitative analysis required further consideration. On the other hand, in LC-UV-MS analysis, baseline separation of all the five components was possible when a mixture of ammonium bicarbonate buffer and acetonitrile was used as the mobile phase. In addition, calibration curves showed adequate linearity with good recovery rates. On the basis of these results, we decided to employ the analytical condition by LC-UVMS. Using this method, we analyzed commercial health food to determine the actual contents of yohimbine and its stereoisomers, and identified food products in which only yohimbine was detected, as well as those in which yohimbine and its four stereoisomers were detected. Furthermore, we found at a high frequency food products in which corynanthine and α-yohimbine were detected at higher concentrations than yohimbine.
  • 島村 智子, 伊藤 裕才, 久保 勇人, 柏木 丈拡, 石川 洋哉, 松井 利郎, 山崎 壮, 多田 敦子, 杉本 直樹, 穐山 浩, 受田 ...
    2017 年 24 巻 1 号 p. 10-15
    発行日: 2017年
    公開日: 2017/04/29
    ジャーナル フリー
    In this study, the relationship between the catechin content and antioxidant capacity of the tea extract, a natural food additive, was investigated to establish the quality standards based on the antioxidant capacity. The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant capacities were detected in 13 kinds of tea extracts among the tested 14 kinds of them. A correlation was found between the total amount of catechins (C, EC, GC, EGC, Cg, ECg, GCg, and EGCg) and the antioxidant capacity (r = 0.975, n = 13, p ‹ 0.01). In addition, the contribution ratio of all kinds of catechins in the tea extract to the antioxidant capacity was 90%. These results suggest that the DPPH assay is a useful method to evaluate the antioxidant capacity in the tea extract for establishing the quality standards.
  • 金岡 秀明, 伊藤 克, 橋川(荒木) 裕子, 矢野 竹男, 奥村 克純, 西村 訓弘, 籠谷 和弘
    2017 年 24 巻 1 号 p. 16-24
    発行日: 2017年
    公開日: 2017/04/29
    ジャーナル フリー
    Ginger has been reported to show many health enhancement effects. We prepared a hexane extract of raw ginger (HRG) under low temperature, and examined its anti-obesity effects using 3T3-L1 cells. HRG promoted adipocyte differentiation in a dosedependent manner and enhanced the expression levels of adipocyte-specific genes, such as adiponectin and Glut4 . HRG also exhibited peroxisome proliferator-activated receptor γ ligand activity, similar to antidiabetic drug thiazolidinediones, which promoted adipocyte differentiation and increased small adipocytes. In addition, HRG showed a tendency to suppress the action of tumor necrosis factor-α, which causes insulin resistance by downregulating the expression of adipocyte-specific genes. These findings suggest that HRG would be utilized as a functional food material for the prevention of obesity and related diseases by increasing small adipocytes.
  • 鈴木 一平, 大槻 崇, 吉田 充哉, 吉田 美佳, 阿部 裕, 久保田 浩樹, 建部 千絵, 多田 敦子, 矢野 竹男, 穐山 浩, 佐藤 ...
    2017 年 24 巻 1 号 p. 25-31
    発行日: 2017年
    公開日: 2017/04/29
    ジャーナル フリー
    Octanoic acid (OA) is an ingredient of peracetic acid-based sanitizers (PAS), which are widely used in the sanitation of uncooked food. Since, OA can remain on PAS-treated foods, we developed a simple analytical method to determine OA levels in uncooked foods. The developed method involves straightforward solvent extraction with diethyl ether, derivatization with sulfuric acid/ methanol and gas chromatography coupled with mass spectrometry. The recovery and relative standard deviation of OA ranged from 74.2 to 96.6% and 2.6 to 9.6%, respectively. The limit of quantification (LOQ) in foods was estimated to be 0.02 mg/kg. We applied the developed method to imported uncooked foods (56 beef samples, 34 vegetable samples and 89 fruit samples), and found that OA levels ranged from 0.34 to 0.53 mg/kg, the LOQ to 0.48 mg/kg and LOQ to 1.12 mg/kg, respectively. Most of the determined OA in imported uncooked foods could be considered to be derived from OA naturally contained in the foods.
  • 政田 さやか, 内山 奈穂子, 合田 幸広, 袴塚 高志
    2017 年 24 巻 1 号 p. 32-38
    発行日: 2017年
    公開日: 2017/04/29
    ジャーナル フリー
    Bilberry fruit extracts have been used as herbal medicines for the treatment of vascular and vision disorders in Europe, whereas they are only used in health food products in Japan. In April 2015, “Foods with Functional Claims (FFCs)” was established as a new category of voluntary labeling in health food products sold in Japan, and several bilberry-containing products have been submitted as FFCs. To ensure the efficacy and safety of the FFCs, effective quality control on the original plants, consistent composition, and manufacturing process is important for the herbal ingredients with specific health-related functions. In this study, we evaluated the quality and quantity of 5 FFCs whose functional substances are bilberry anthocyanins. Twenty compounds (15 anthocyanins and 5 anthocyanidins) in the FFCs were separated by the HPLC method according to the European Pharmacopoeia instead of the journal featuring dietary supplements. Cyanidin-3-rutinoside was detected in 3 FFCs containing black currant extracts as well as bilberry extracts, and the anthocyanins in these FFCs were considered to be derived from both plants. Since the bilberry extracts are the active ingredient with pharmaceutical evidence and its health benefits are displayed on the product’s packaging, the proper regulation for ensuring a consistent quality of bilberry-containing FFCs would be recommended.
ノート
  • 間瀬 民生, 下末 祥代, 近藤 徹弥, 田中 万絢, 高田 莉帆, 日渡 美世, 一色 忍, 河合 潤子
    2017 年 24 巻 1 号 p. 39-43
    発行日: 2017年
    公開日: 2017/04/29
    ジャーナル フリー
    A newly isolated yeast strain, Pseudozyma hubeiensis 31-B, produced an extracellular carboxypeptidase but no detectable proteinase activity. The carboxypeptidase was purified from filtered culture medium by ammonium sulfate precipitation and successive four column chromatography steps: TOYOPEARL DEAE-650M, TOYOPEARL Butyl-650M, hydroxylapatite, and TOYOPEARL HW-55 chromatography. The final enzyme preparation appeared homogeneous on SDS-polyacrylamide gel electrophoresis. The enzyme had a molecular mass of 70.5 kDa. The optimum temperature and pH of the purified enzyme were approximately 50°C and 5.0, respectively. The purified carboxypeptidase preferentially hydrolyzed Z-Phe-Leu, and its activity was inhibited by diisopropyl fluorophosphate. The carboxypeptidase produced by P. hubeiensis 31-B has potential applications in the food industry as a food additive.
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